PMID:9367992

Citation	Li, L, Ernsting, BR, Wishart, MJ, Lohse, DL and Dixon, JE (1997) A family of putative tumor suppressors is structurally and functionally conserved in humans and yeast. J. Biol. Chem. 272:29403-6
Abstract	In Saccharomyces cerevisiae the CDC14 gene is essential for cell cycle progression. Strains carrying the cdc14-1(ts) allele enter the cell cycle and arrest at restrictive temperatures. We have identified two human cDNAs encoding proteins which share sequence identity to the yeast CDC14p. The cell cycle arrest in cdc14-1(ts) can be specifically complemented by the human cDNAs suggesting that they are functionally equivalent to the yeast CDC14 gene. Another clone identified in the search for human CDC14-like proteins corresponded to the putative tumor suppressor gene PTEN/MMAC1 (phosphatase and tensin homologue deleted on chromosome 10 or mutated in multiple advanced cancers 1). Analysis of the PTEN/MMAC1 showed that it did not complement the cdc14-1(ts) allele and that it is more closely related to the yeast open reading frame YNL128W. Human CDC14p and PTEN/MMAC1 were expressed as recombinant proteins, and both were shown to have kinetic properties characteristic of dual specific phosphatases. The human CDC14p was localized in the nucleus while PTEN/MMAC1 has been reported to be localized in the cytoplasm. Our results suggest that CDC14 and YNL128W/PTEN/MMAC1 represent two related, but distinct, families of human and yeast phosphatases.
Links	PubMed
Keywords	Alleles; Amino Acid Sequence; Binding Sites; Cell Compartmentation; Cell Cycle; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/genetics; Conserved Sequence; DNA, Complementary/chemistry; Fungal Proteins/chemistry; Fungal Proteins/genetics; Humans; Kinetics; Molecular Sequence Data; Myocardium/chemistry; Open Reading Frames; PTEN Phosphohydrolase; Phosphoric Monoester Hydrolases; Protein Tyrosine Phosphatases/chemistry; Protein Tyrosine Phosphatases/genetics; Saccharomyces cerevisiae/chemistry; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Sequence Alignment; Tumor Suppressor Proteins
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:CC14B	located_in	GO:0005634: nucleus	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
HUMAN:CC14B	enables	GO:0004722: protein serine/threonine phosphatase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:CC14B	enables	GO:0004725: protein tyrosine phosphatase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:CC14B	involved_in	GO:0006470: protein dephosphorylation	ECO:0000303: author statement without traceable support used in manual assertion		P		Seeded From UniProt	complete
HUMAN:PTEN	located_in	GO:0005737: cytoplasm	ECO:0000304: author statement supported by traceable reference used in manual assertion		C		Seeded From UniProt	complete
HUMAN:PTEN	involved_in	GO:0006470: protein dephosphorylation	ECO:0000304: author statement supported by traceable reference used in manual assertion		P		Seeded From UniProt	complete
HUMAN:PTEN	part_of	GO:0005737: cytoplasm	ECO:0000304: author statement supported by traceable reference used in manual assertion		C		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.