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PMID:9346235

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Citation

Rieckhof, GE, Casares, F, Ryoo, HD, Abu-Shaar, M and Mann, RS (1997) Nuclear translocation of extradenticle requires homothorax, which encodes an extradenticle-related homeodomain protein. Cell 91:171-83

Abstract

We show that homothorax (hth) is required for the Hox genes to pattern the body of the fruit fly, Drosophila melanogaster. hth is necessary for the nuclear localization of an essential HOX cofactor, Extradenticle (EXD), and encodes a homeodomain protein that shares extensive identity with the product of Meis1, a murine proto-oncogene. MEIS1 is able to rescue hth mutant phenotypes and can induce the cytoplasmic-to-nuclear translocation of EXD in cell culture and Drosophila embryos. Thus, Meis1 is a murine homolog of hth. MEIS1/HTH also specifically binds to EXD with high affinity in vitro. These data suggest a novel and evolutionarily conserved mechanism for regulating HOX activity in which a direct protein-protein interaction between EXD and HTH results in EXD's nuclear translocation.

Links

PubMed

Keywords

Animal Structures/physiology; Animals; Biological Transport/genetics; Cell Nucleus/chemistry; Cell Nucleus/metabolism; Cytoplasm/chemistry; DNA-Binding Proteins/analysis; DNA-Binding Proteins/metabolism; Drosophila Proteins; Drosophila melanogaster/genetics; Embryo, Nonmammalian/physiology; Embryonic and Fetal Development/physiology; Gene Expression Regulation, Developmental; Genes, Homeobox/physiology; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Leukemia, Myeloid; Mice; Molecular Sequence Data; Neoplasm Proteins/genetics; Neoplasm Proteins/metabolism; Sequence Homology, Amino Acid; Transcription Factors/analysis; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:HTH

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0000611

F

Seeded From UniProt

complete

DROME:HTH

involved_in

GO:0034504: protein localization to nucleus

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0000611

P

Seeded From UniProt

complete

DROME:HTH

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:HTH

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P40427

F

Seeded From UniProt

complete

DROME:HTH

part_of

GO:0005667: transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:EXD

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0001235

F

Seeded From UniProt

complete

DROME:EXD

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:EXD

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:EXD

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O46339

F

Seeded From UniProt

complete


See also

References

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