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PMID:9326634

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Citation

Woodage, T, Basrai, MA, Baxevanis, AD, Hieter, P and Collins, FS (1997) Characterization of the CHD family of proteins. Proc. Natl. Acad. Sci. U.S.A. 94:11472-7

Abstract

The murine gene CHD1 (MmCHD1) was previously isolated in a search for proteins that bound a DNA promoter element. The presence of chromo (chromatin organization modifier) domains and an SNF2-related helicase/ATPase domain led to speculation that this gene regulated chromatin structure or gene transcription. This study describes the cloning and characterization of three novel human genes related to MmCHD1. Examination of sequence databases produced several more related genes, most of which were not known to be similar to MmCHD1, yielding a total of 12 highly conserved CHD genes from organisms as diverse as yeast and mammals. The major region of sequence variation is in the C-terminal part of the protein, a region with DNA-binding activity in MmCHD1. Targeted deletion of ScCHD1, the sole Saccharomyces cerevesiae CHD gene, was performed with deletion strains being less sensitive than wild type to the cytotoxic effect of 6-azauracil. This finding suggested that enhanced transcriptional arrest at RNA polymerase II pause sites due to 6-azauracil-induced nucleotide pool depletion was reduced in the deletion strain and that ScCHD1 inhibited transcription. This observation, along with the known roles of other proteins with chromo or SNF2-related helicase/ATPase domains, suggests that alteration of gene expression by CHD genes might occur by modifications of chromatin structure, with altered access of the transcriptional apparatus to its chromosomal DNA template.

Links

PubMed PMC23509

Keywords

Adenosine Triphosphatases/chemistry; Amino Acid Sequence; Animals; Chromatin/physiology; Chromosome Mapping; Cloning, Molecular; DNA Helicases/chemistry; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; Evolution, Molecular; Humans; Mammals; Mice; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction; Recombinant Proteins/biosynthesis; Recombinant Proteins/chemistry; Sequence Alignment; Sequence Homology, Amino Acid; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:CHD1

is_active_in

GO:0000228: nuclear chromosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CHD2

enables

GO:0003677: DNA binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

DROME:CHD3

involved_in

GO:0006333: chromatin assembly or disassembly

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CHD3

enables

GO:0003677: DNA binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:CHD4

enables

GO:0003677: DNA binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:CHD1

involved_in

GO:0006357: regulation of transcription by RNA polymerase II

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CHD1

enables

GO:0004003: ATP-dependent DNA helicase activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete


See also

References

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