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PMID:9305894

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Citation

Muta, T, Kang, D, Kitajima, S, Fujiwara, T and Hamasaki, N (1997) p32 protein, a splicing factor 2-associated protein, is localized in mitochondrial matrix and is functionally important in maintaining oxidative phosphorylation. J. Biol. Chem. 272:24363-70

Abstract

Human p32, originally cloned as a splicing factor 2-associated protein, has been reported to interact with a variety of molecules including human immunodeficiency virus Tat and complement 1q (C1q). p32 protein is supposed to be in the nucleus and on the plasma membrane for the association with human immunodeficiency virus Tat and C1q, respectively. None of the interactions, however, is proven to have a physiological role. To investigate the physiological function of p32, we determined the intracellular localization of p32. The fractionation of cells, fluorescent immunocytochemistry, and electron microscopic immunostaining show that p32 is exclusively localized in the mitochondrial matrix. We cloned a Saccharomyces cerevisiae homologue of human p32 gene, referred to yeast p30 gene. The yeast p30 protein is also localized in the mitochondrial matrix. The disruption of the p30 gene caused the growth retardation of yeast cells in a glycerol medium but not in a glucose medium, i.e. the impairment of the mitochondrial ATP synthesis. The growth impairment was restored by the introduction of the human p32 cDNA, indicating that p30 is a functional yeast counterpart of human p32. Taken together, both p32 and p30 reside in mitochondrial matrix and play an important role in maintaining mitochondrial oxidative phosphorylation.

Links

PubMed

Keywords

Amino Acid Sequence; Antigens, CD44; Carrier Proteins; Cell Line; Cell Nucleus/metabolism; Gene Targeting; Humans; Immunohistochemistry; Mitochondria/metabolism; Mitochondrial Proteins; Molecular Sequence Data; Oxidative Phosphorylation; Proteins/metabolism; RNA Splicing; Saccharomyces cerevisiae/genetics; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:MAM33

located_in

GO:0005759: mitochondrial matrix

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:MAM33

involved_in

GO:0009060: aerobic respiration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:C1QBP

GO:0005759: mitochondrial matrix

ECO:0000314:

C

Figure 3 and Table II help illustrate how p32 is found in the mitochondrial matrix. On the basis of recovery of fumarase activity in the mitoplast fraction, the amount of p32 in mitoplasts accounted for about 102% p32 in intact mitochondria. Complement component 1 Q subcomponent-binding protein, mitochondrial Human

complete
CACAO 12793

YEAST:UAF30

GO:0005759: mitochondrial matrix

ECO:0000314:

C

Figure 6 shows that like human p32, p30 is also localized in the mitochondrial matrix. Upstream activation factor subunit UAF30 Saccharomyces cerevisiae

complete
CACAO 12794

YEAST:UAF30

GO:0016049: cell growth

ECO:0000315:

P

Disruption of the p30 gene in yeast caused growth retardation in the glycerol medium, as seen in Figure 7. Thus it can be inferred that p30 is important for cell growth Upstream activation factor subunit UAF30 Saccharomyces cerevisiae

complete
CACAO 12795

Notes

See also

References

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