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PMID:9299423

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Citation

Kume, K and Shimizu, T (1997) cDNA cloning and expression of murine 1-acyl-sn-glycerol-3-phosphate acyltransferase. Biochem. Biophys. Res. Commun. 237:663-6

Abstract

1-Acyl-sn-glycerol-3-phosphate (1-AGP), also known as lysophosphatidic acid (LPA), is an intermediate of de novo biosynthesis of glycerophospholipids and triacylglycerol. LPA is also attracting much attention because of its growth stimulating effects. Here we report cloning of murine cDNA encoding 1-AGP acyltransferase (1-AGPAT), which converts LPA into phosphatidic acid by incorporating acyl moiety at an-2 position. The cDNA contains an open reading frame coding for 285 amino acids, with highly hydrophobic regions in the N-terminal half. The Northern blot analysis using various mouse tissues revealed ubiquitous expressions of two transcripts. The cDNA was expressed in Escherichia coli JC201, a plsC mutant strain deficient in the 1-AGPAT activity, and the enzyme properties were examined. The enzyme utilized both saturated and unsaturated acyl-CoA as an acyl-donor, while it utilized LPA but not other lysophospholipids as an acceptor.

Links

PubMed Online version:10.1006/bbrc.1997.7214

Keywords

1-Acylglycerol-3-Phosphate O-Acyltransferase; Acyltransferases/biosynthesis; Acyltransferases/chemistry; Acyltransferases/metabolism; Amino Acid Sequence; Animals; Base Sequence; Brain/enzymology; Cloning, Molecular; DNA, Complementary/biosynthesis; Escherichia coli/enzymology; Escherichia coli Proteins; Humans; Mice; Molecular Sequence Data; Open Reading Frames; Recombinant Proteins/biosynthesis; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Saccharomyces cerevisiae/enzymology; Sequence Alignment; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:PLCA

acts_upstream_of_or_within

GO:0006654: phosphatidic acid biosynthetic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:PLCA

enables

GO:0003841: 1-acylglycerol-3-phosphate O-acyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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