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PMID:9287002

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Citation

Lommatzsch, J, Templin, MF, Kraft, AR, Vollmer, W and Höltje, JV (1997) Outer membrane localization of murein hydrolases: MltA, a third lipoprotein lytic transglycosylase in Escherichia coli. J. Bacteriol. 179:5465-70

Abstract

Lytic transglycosylases are a unique lysozyme-like class of murein hydrolases believed to be important for growth of Escherichia coli. A membrane-bound lytic transglycosylase with an apparent molecular mass of 38 kDa, which was designated Mlt38, has previously been purified and characterized (A. Ursinus and J.-V. Höltje, J. Bacteriol. 176:338-343, 1994). On the basis of four tryptic peptides, the gene mltA was mapped at 63 min on the chromosomal map of E. coli K-12 and cloned by reverse genetics. The open reading frame was found to contain a typical lipoprotein consensus sequence, and the lipoprotein nature of the gene product was demonstrated by [3H]palmitate labeling. On the basis of the distribution of MltA in membrane fractions obtained by sucrose gradient centrifugation, a localization in the outer membrane is indicated. Overexpression of MltA at 30 degrees C, the optimal temperature for enzyme activity, but not at 37 degrees C results in the formation of spheroplasts. Not only a deletion mutant in mltA, but also double mutants in mltA and one of the two other well-characterized lytic transglycosylases (either sltY or mltB), as well as a triple mutant in all three enzymes, showed no obvious phenotype. However, dramatic changes in the structure of the murein sacculus indicate that lytic transglycosylases are involved in maturation of the murein sacculus.

Links

PubMed PMC179418

Keywords

Amino Acid Sequence; Base Sequence; Cell Membrane/enzymology; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial/genetics; Glycosyltransferases/analysis; Glycosyltransferases/chemistry; Glycosyltransferases/genetics; Lipoproteins/analysis; Lipoproteins/chemistry; Lipoproteins/genetics; Molecular Sequence Data; Open Reading Frames/genetics; Peptide Fragments/genetics; Peptidoglycan/analysis; Restriction Mapping; Sequence Analysis, DNA

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:MLTA

located_in

GO:0036405: anchored component of cell outer membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:MLTA

located_in

GO:0009279: cell outer membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:SLT

GO:0071555: cell wall organization

ECO:0000316:

UniProtKB:P0A908 UniProtKB:P41052


P

Table 2

complete
CACAO 3787

ECOLI:MLTA

GO:0009279: cell outer membrane

ECO:0000314:

C

Figure 4 Membrane fractionation by sucrose gradient centrifugation shows that the MltA protein is also localized in the outer membrane

complete
CACAO 12190

ECOLI:MLTA

part_of

GO:0009279: cell outer membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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