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PMID:9271107

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Citation

Türkel, S, Liao, XB and Farabaugh, PJ (1997) GCR1-dependent transcriptional activation of yeast retrotransposon Ty2-917. Yeast 13:917-30

Abstract

Transcription of Saccharomyces cerevisiae Ty2-917 retrotransposon depends on regulatory elements both upstream and downstream of the transcription initiation site. An upstream activation sequence (UAS) and a downstream enhancer stimulate transcription synergistically. Here we show that activation by both of these sites depends on the GCR1 product, a transcription factor which also regulates the genes encoding yeast glycolytic enzymes. Eliminating GCR1 causes a 100-fold decrease in transcription of Ty2-917. Activation by the isolated Ty2-917 UAS also strongly depends on GCR1. Unexpectedly, GCR1-dependent activation by the Ty2-917 enhancer is strongly position-dependent. Activation by the enhancer in its normal position within the transcription unit depended strongly on GCR1, but eliminating GCR1 reduced activation only three-fold when the enhancer was moved upstream of the transcribed region. Gel mobility shift and DNaseI protection assays indicated that GCR1 binds specifically to multiple sites within the Ty2-917 UAS and enhancer regions.

Links

PubMed Online version:<917::AID-YEA148>3.0.CO;2-G 10.1002/(SICI)1097-0061(199708)13:10<917::AID-YEA148>3.0.CO;2-G

Keywords

Base Sequence; DNA/metabolism; DNA-Binding Proteins/physiology; Enhancer Elements, Genetic; Fungal Proteins/physiology; Molecular Sequence Data; Retroelements; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Transcription Factors; Transcriptional Activation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:GCR1

enables

GO:0000978: RNA polymerase II cis-regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:GCR1

enables

GO:0001228: DNA-binding transcription activator activity, RNA polymerase II-specific

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:GCR1

involved_in

GO:0061435: positive regulation of transcription from a mobile element promoter

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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