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PMID:9260967

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Citation

Seta, FD, Boschi-Muller, S, Vignais, ML and Branlant, G (1997) Characterization of Escherichia coli strains with gapA and gapB genes deleted. J. Bacteriol. 179:5218-21

Abstract

We obtained a series of Escherichia coli strains in which gapA, gapB, or both had been deleted. Delta gapA strains do not revert on glucose, while delta gapB strains grow on glycerol or glucose. We showed that gapB-encoded protein is expressed but at a very low level. Together, these results confirm the essential role for gapA in glycolysis and show that gapB is dispensable for both glycolysis and the pyridoxal biosynthesis pathway.

Links

PubMed PMC179383

Keywords

Aldehyde Oxidoreductases/genetics; Aldehyde Oxidoreductases/metabolism; Culture Media; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Deletion; Glucose/metabolism; Glyceraldehyde-3-Phosphate Dehydrogenases/genetics; Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism; Glycerol/metabolism; Glycolysis; Phenotype; Pyridoxal Phosphate/biosynthesis; Succinates/metabolism; Succinic Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:G3P1

acts_upstream_of_or_within

GO:0006096: glycolytic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:G3P1

GO:0004365: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity

ECO:0000315:

F

Table 3. When a phage is inserted into gapA no cell growth is detected, due to the lack of GAPDHase activity.

complete

ECOLI:G3P1

GO:0006096: glycolysis

ECO:0000315:

P

Table 3. When a phage was inserted into gapA the E. coli strain was inable to grow on glucose. Expression of gapA completely restored this ability confirming it's role in glycolysis.

complete

ECOLI:G3P1

involved_in

GO:0006096: glycolytic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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