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PMID:9241251

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Citation

Benyajati, C, Mueller, L, Xu, N, Pappano, M, Gao, J, Mosammaparast, M, Conklin, D, Granok, H, Craig, C and Elgin, S (1997) Multiple isoforms of GAGA factor, a critical component of chromatin structure. Nucleic Acids Res. 25:3345-53

Abstract

The GAGA transcription factor of Drosophila melanogaster is ubiquitous and plays multiple roles. Characterization of cDNA clones and detection by domain- specific antibodies has revealed that the 70-90 kDa major GAGA species are encoded by two open reading frames producing GAGA factor proteins of 519 amino acids (GAGA-519) and 581 amino acids (GAGA-581), which share a common N-terminal region that is linked to two different glutamine-rich C-termini. Purified recombinant GAGA-519 and GAGA-581 proteins can form homomeric complexes that bind specifically to a single GAGA sequence in vitro. The two GAGA isoforms also function similarly in transient transactivation assays in tissue culture cells and in chromatin remodeling experiments in vitro . Only GAGA-519 protein accumulates during the first 6 h of embryogenesis. Thereafter, both GAGA proteins are present in nearly equal amounts throughout development; in larval salivary gland nuclei they colocalize completely to specific regions along the euchromatic arms of the polytene chromosomes. Coimmunoprecipitation of GAGA-519 and GAGA-581 from crude nuclear extracts and from mixtures of purified recombinant proteins, indicates direct interactions. We suggest that homomeric complexes of GAGA-519 may function during early embryogenesis; both homomeric and heteromeric complexes of GAGA-519 and GAGA-581 may function later.

Links

PubMed PMC146888

Keywords

Amino Acid Sequence; Animals; Base Sequence; Chromatin/ultrastructure; Cloning, Molecular; DNA, Complementary/genetics; DNA-Binding Proteins/chemistry; Drosophila Proteins; Drosophila melanogaster; Gene Expression Regulation, Developmental; Homeodomain Proteins/chemistry; Insect Proteins/genetics; Insect Proteins/metabolism; Molecular Sequence Data; Molecular Weight; Nucleoproteins/chemistry; Nucleosomes/ultrastructure; Protein Binding; Transcription Factors/chemistry

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:GAGA

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:GAGA

located_in

GO:0000791: euchromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:GAGA

located_in

GO:0005700: polytene chromosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:GAGA

involved_in

GO:0031497: chromatin assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:GAGA

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:GAGA

enables

GO:0046982: protein heterodimerization activity

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q08605

F

Seeded From UniProt

complete

DROME:GAGA

involved_in

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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