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PMID:9214622

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Citation

Watanabe, N, Madaule, P, Reid, T, Ishizaki, T, Watanabe, G, Kakizuka, A, Saito, Y, Nakao, K, Jockusch, BM and Narumiya, S (1997) p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin. EMBO J. 16:3044-56

Abstract

Rho small GTPase regulates cell morphology, adhesion and cytokinesis through the actin cytoskeleton. We have identified a protein, p140mDia, as a downstream effector of Rho. It is a mammalian homolog of Drosophila diaphanous, a protein required for cytokinesis, and belongs to a family of formin-related proteins containing repetitive polyproline stretches. p140mDia binds selectively to the GTP-bound form of Rho and also binds to profilin. p140mDia, profilin and RhoA are co-localized in the spreading lamellae of cultured fibroblasts. They are also co-localized in membrane ruffles of phorbol ester-stimulated sMDCK2 cells, which extend these structures in a Rho-dependent manner. The three proteins are recruited around phagocytic cups induced by fibronectin-coated beads. Their recruitment is not induced after Rho is inactivated by microinjection of botulinum C3 exoenzyme. Overexpression of p140mDia in COS-7 cells induced homogeneous actin filament formation. These results suggest that Rho regulates actin polymerization by targeting profilin via p140mDia beneath the specific plasma membranes.

Links

PubMed PMC1169923 Online version:10.1093/emboj/16.11.3044

Keywords

3T3 Cells; Amino Acid Sequence; Animals; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Adhesion; Cell Membrane/metabolism; Cell Movement; Contractile Proteins; Drosophila/genetics; Drosophila Proteins; Fluorescent Antibody Technique; Fungal Proteins/genetics; GTP-Binding Proteins/metabolism; Insect Proteins/genetics; Ligands; Mice; Microfilament Proteins/metabolism; Molecular Sequence Data; Profilins; Protein Binding; Recombinant Proteins/metabolism; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; rhoA GTP-Binding Protein

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:DIAP1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q9QUI0

F

Seeded From UniProt

complete

MOUSE:DIAP1

enables

GO:0005522: profilin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:DIAP1

acts_upstream_of_or_within

GO:0030036: actin cytoskeleton organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DIAP1

enables

GO:0031267: small GTPase binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(MGI:MGI:1096342)

Seeded From UniProt

complete

MOUSE:DIAP1

located_in

GO:0032587: ruffle membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:DIAP1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P61586

F

Seeded From UniProt

complete

HUMAN:RHOA

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O08808

F

Seeded From UniProt

complete

MOUSE:RHOA

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:O08808

F

Seeded From UniProt

complete

MOUSE:RHOA

located_in

GO:0032587: ruffle membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:RHOA

part_of

GO:0032587: ruffle membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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