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PMID:9211901

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Citation

Murao, K, Terpstra, V, Green, SR, Kondratenko, N, Steinberg, D and Quehenberger, O (1997) Characterization of CLA-1, a human homologue of rodent scavenger receptor BI, as a receptor for high density lipoprotein and apoptotic thymocytes. J. Biol. Chem. 272:17551-7

Abstract

Recently, a murine scavenger receptor type B class I (SR-BI) was identified that binds high density lipoprotein (HDL) and mediates the selective uptake of cholesterol esters. The human CD36 and LIMPII analogous-1 (CLA-1) receptor shows high sequence homology with SR-BI, but their functional relationship has not been determined. Transfected cells expressing CLA-1 bound HDL with a Kd of about 35 microg/ml, similar to the Kd for HDL binding to rodent SR-BI. This binding resulted in an intracellular accumulation of HDL-derived [3H]cholesterol esters without internalization or degradation of 125I-apolipoprotein. CLA-1 was strongly expressed in the adrenal gland and was also abundant in liver and testis, suggesting that CLA-1, like SR-BI, could play a role in the metabolism of HDL. However, CLA-1 was also expressed in monocytes and, like SR-BI, recognized modified forms of low density lipoproteins as well as native LDL and anionic phospholipids. These findings suggest that CLA-1 might have additional physiological functions. We found that CLA-1 recognizes apoptotic thymocytes. Our results demonstrate that CLA-1, a close structural homologue of SR-BI, is also functionally related to SR-BI and may play an important role as a "docking receptor" for HDL in connection with selective uptake of cholesterol esters. An additional role in recognition of damaged cells is suggested by these studies.

Links

PubMed

Keywords

Animals; Antigens, CD36/genetics; Antigens, CD36/metabolism; Apoptosis; Blotting, Western; Carrier Proteins; Cell Line; Cholesterol Esters/metabolism; Humans; Ligands; Lipoproteins, HDL/metabolism; Membrane Proteins; Mice; RNA-Binding Proteins; Receptors, Immunologic; Receptors, Lipoprotein/metabolism; Receptors, Scavenger; Scavenger Receptors, Class B; Thymus Gland/cytology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:SCRB1

enables

GO:0030169: low-density lipoprotein particle binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q8WTV0

F

Seeded From UniProt

complete

MOUSE:SCRB1

involved_in

GO:0034384: high-density lipoprotein particle clearance

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q8WTV0

P

Seeded From UniProt

complete

MOUSE:SCRB1

involved_in

GO:0010886: positive regulation of cholesterol storage

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q8WTV0

P

Seeded From UniProt

complete

MOUSE:SCRB1

located_in

GO:0005886: plasma membrane

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q8WTV0

C

Seeded From UniProt

complete

MOUSE:SCRB1

enables

GO:0070506: high-density lipoprotein particle receptor activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q8WTV0

F

Seeded From UniProt

complete

MOUSE:SCRB1

involved_in

GO:0043654: recognition of apoptotic cell

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q8WTV0

P

Seeded From UniProt

complete

HUMAN:SCRB1

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:SCRB1

enables

GO:0070506: high-density lipoprotein particle receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:SCRB1

involved_in

GO:0050764: regulation of phagocytosis

ECO:0000305: curator inference used in manual assertion

GO:0043654

P

Seeded From UniProt

complete

HUMAN:SCRB1

involved_in

GO:0043691: reverse cholesterol transport

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SCRB1

involved_in

GO:0043654: recognition of apoptotic cell

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SCRB1

involved_in

GO:0034384: high-density lipoprotein particle clearance

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SCRB1

enables

GO:0030169: low-density lipoprotein particle binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:SCRB1

enables

GO:0005545: 1-phosphatidylinositol binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:SCRB1

enables

GO:0005215: transporter activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:SCRB1

involved_in

GO:0010886: positive regulation of cholesterol storage

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SCRB1

part_of

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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