PMID:9119474

Citation	Molinari, G, Talay, SR, Valentin-Weigand, P, Rohde, M and Chhatwal, GS (1997) The fibronectin-binding protein of Streptococcus pyogenes, SfbI, is involved in the internalization of group A streptococci by epithelial cells. Infect. Immun. 65:1357-63
Abstract	Streptococcus pyogenes organisms (group A streptococci) are considered to be highly adhesive extracellular pathogens. However, it has recently been reported that S. pyogenes has the capacity to efficiently invade eukaryotic cells. In this study, we demonstrate that the interaction of S. pyogenes fibronectin-binding protein (SfbI) with fibronectin on nonphagocytic HEp-2 cells triggers bacterial internalization. Blocking of the SfbI adhesin by either antibodies against the whole protein or antibodies against the fibronectin-binding domains of SfbI, as well as pretreatment of HEp-2 cells with purified SfbI protein, prevents both S. pyogenes attachment and internalization. Inert latex beads precoated with the purified SfbI protein are ingested by eukaryotic cells, demonstrating that SfbI is per se enough to trigger the internalization process. Experiments performed with a recombinant SfbI domain encompassing the two fibronectin-binding regions of the SfbI molecule demonstrated that these binding regions are essential and sufficient to activate uptake by HEp-2 cells. These results demonstrate that the fibronectin-binding protein SfbI is involved in both S. pyogenes' attachment to and ingestion by HEp-2 cells and contribute to elucidation of the underlying molecular events leading to eukaryotic cell invasion by S. pyogenes.
Links	PubMed PMC175140
Keywords	Adhesins, Bacterial; Bacterial Adhesion/physiology; Bacterial Proteins/physiology; Carrier Proteins/physiology; Cells, Cultured; Epithelium/microbiology; Humans; Microscopy, Electron; Streptococcal Infections/microbiology; Streptococcus pyogenes/physiology; Streptococcus pyogenes/ultrastructure
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
STRPY:Q711B0		GO:0009405: pathogenesis	ECO:0000314:		P		Figure 6. shows electron microscopic analysis of SfbI coated latex bead uptake by HEp-2 cells.	complete CACAO 5258
STRPY:Q711B0	involved_in	GO:0009405: pathogenesis	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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