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PMID:9119111

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Citation

Ng, LJ, Wheatley, S, Muscat, GE, Conway-Campbell, J, Bowles, J, Wright, E, Bell, DM, Tam, PP, Cheah, KS and Koopman, P (1997) SOX9 binds DNA, activates transcription, and coexpresses with type II collagen during chondrogenesis in the mouse. Dev. Biol. 183:108-21

Abstract

Two lines of evidence suggest that the Sry-related gene Sox9 is important for chondrogenesis in mammalian embryos. Sox9 mRNA is expressed in chondrogenic condensations in mice, and mutations in human SOX9 are known to cause skeletal dysplasia. We show here that mouse SOX9 protein is able to bind to a SOX/SRY consensus motif in DNA and contains a modular transcriptional activation domain, consistent with a role for SOX9 as a transcription factor acting on genes involved in cartilage development. One such gene is Col2a1, which encodes type II collagen, the major structural component of cartilage. We have compared, in detail, the expression of Sox9 and Col2a1 during mouse development. In chondrogenic tissues the expression profiles of the two genes were remarkably similar. Coexpression was detected in some nonchondrogenic tissues such as the notochord, otic vesicle, and neural tube, but others such as heart and lung differed in their expression of the two genes. Immunohistochemistry using an antibody specific for SOX9 revealed that expression of SOX9 protein mirrored the distribution of Sox9 mRNA. Our results suggest that SOX9 protein is involved in the regulation of Col2a1 during chondrogenesis, but that this regulation is likely to depend on additional cofactors.

Links

PubMed Online version:10.1006/dbio.1996.8487

Keywords

Animals; COS Cells; Cartilage/embryology; Collagen/genetics; Consensus Sequence/genetics; DNA/metabolism; DNA-Binding Proteins/metabolism; Embryonic and Fetal Development; Gene Expression Regulation, Developmental/physiology; Germ Layers/chemistry; High Mobility Group Proteins/analysis; High Mobility Group Proteins/genetics; High Mobility Group Proteins/metabolism; Mice; Nuclear Proteins; Organ Specificity; RNA, Messenger/analysis; SOX9 Transcription Factor; Sequence Deletion; Sex-Determining Region Y Protein; Transcription Factors/analysis; Transcription Factors/genetics; Transcription Factors/metabolism; Transcriptional Activation/physiology; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CO2A1

involved_in

GO:0002062: chondrocyte differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CO2A1

involved_in

GO:0001503: ossification

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CO2A1

involved_in

GO:0007417: central nervous system development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CO2A1

involved_in

GO:0030903: notochord development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CO2A1

involved_in

GO:0071599: otic vesicle development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SOX9

involved_in

GO:0002062: chondrocyte differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SOX9

involved_in

GO:0001503: ossification

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SOX9

involved_in

GO:0007417: central nervous system development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SOX9

involved_in

GO:0030903: notochord development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SOX9

enables

GO:0000976: transcription cis-regulatory region binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:SOX9

involved_in

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SOX9

involved_in

GO:0071599: otic vesicle development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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