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PMID:9111338

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Citation

Minoguchi, S, Taniguchi, Y, Kato, H, Okazaki, T, Strobl, LJ, Zimber-Strobl, U, Bornkamm, GW and Honjo, T (1997) RBP-L, a transcription factor related to RBP-Jkappa. Mol. Cell. Biol. 17:2679-87

Abstract

RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. Although at least four Notch genes exist in mammals, it is unknown whether each Notch requires a specific downstream signalling molecule. Here we report isolation and characterization of a mouse RBP-Jkappa-related gene named RBP-L that is expressed almost exclusively in lung, in contrast to the ubiquitous expression of RBP-Jkappa. For simplicity, we propose to call RBP-Jkappa RBP-J. The RBP-L protein bound to a DNA sequence almost identical to that of RBP-J. Surprisingly, RBP-L did not interact with any of the known four mouse Notch proteins. Although we found that RBP-L and EBNA-2 cooperated in transcriptional activation, they did not show significantly strong protein-protein interaction that can be detected by several in vivo and in vitro assays. This is again in contrast to physical association of RBP-J with EBNA-2. Several models to explain functional interaction between RBP-L and EBNA-2 are discussed.

Links

PubMed PMC232118

Keywords

Amino Acid Sequence; Animals; Base Sequence; COS Cells; Cloning, Molecular; DNA/metabolism; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; Epstein-Barr Virus Nuclear Antigens/metabolism; Fungal Proteins/metabolism; Gene Expression Regulation; Immunoglobulin J Recombination Signal Sequence-Binding Protein; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Molecular Sequence Data; Nuclear Proteins; Receptors, Notch; Saccharomyces cerevisiae Proteins; Transcription Factors; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:RBPJL

enables

GO:0000977: RNA polymerase II transcription regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:RBPJL

enables

GO:0001228: DNA-binding transcription activator activity, RNA polymerase II-specific

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:RBPJL

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:RBPJL

located_in

GO:0005634: nucleus

ECO:0000305: curator inference used in manual assertion

GO:0045893

C

Seeded From UniProt

complete

MOUSE:RBPJL

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:RBPJL

acts_upstream_of_or_within

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:NOTC2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P31266

F

Seeded From UniProt

complete

MOUSE:SUH

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:O35516
PR:P31695
PR:Q01705
PR:Q61982

F

Seeded From UniProt

complete

MOUSE:SUH

located_in

GO:0005634: nucleus

ECO:0000305: curator inference used in manual assertion

GO:0045892

C

Seeded From UniProt

complete

MOUSE:SUH

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:SUH

acts_upstream_of_or_within

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:NOTC4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P31266

F

Seeded From UniProt

complete

MOUSE:NOTC1

enables

GO:0003713: transcription coactivator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:NOTC1

acts_upstream_of_or_within

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:NOTC3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P31266

F

Seeded From UniProt

complete


See also

References

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