GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:9109444

From GONUTS
Jump to: navigation, search
Citation

Guerrero, PA, Schuessler, RB, Davis, LM, Beyer, EC, Johnson, CM, Yamada, KA and Saffitz, JE (1997) Slow ventricular conduction in mice heterozygous for a connexin43 null mutation. J. Clin. Invest. 99:1991-8

Abstract

To characterize the role of the gap junction protein connexin43 (Cx43) in ventricular conduction, we studied hearts of mice with targeted deletion of the Cx43 gene. Mice homozygous for the Cx43 null mutation (Cx43 -/-) die shortly after birth. Attempts to record electrical activity in neonatal Cx43 -/- hearts (n = 5) were unsuccessful. Ventricular epicardial conduction of paced beats, however, was 30% slower in heterozygous (Cx43 -/+) neonatal hearts (0.14+/-0.04 m/s, n = 27) than in wild-type (Cx43 +/+) hearts (0.20+/-0.07 m/s, n = 32; P < 0.001). This phenotype was even more severe in adult mice; ventricular epicardial conduction was 44% slower in 6-9 mo-old Cx43 -/+ hearts (0.18+/-0.03 m/s, n = 5) than in wild-type hearts (0.32+/-0.07 m/s, n = 7, P < 0.001). Electrocardiograms revealed significant prolongation of the QRS complex in adult Cx43 -/+ mice (13.4+/-1.8 ms, n = 13) compared with Cx43 +/+ mice (11.5+/-1.4 ms, n = 12, P < 0.01). Whole-cell recordings of action potential parameters in cultured disaggregated neonatal ventricular myocytes from Cx43 -/+ and +/+ hearts showed no differences. Thus, reduction in the abundance of a major cardiac gap junction protein through targeted deletion of a Cx43 allele directly leads to slowed ventricular conduction.

Links

PubMed PMC508024 Online version:10.1172/JCI119367

Keywords

Action Potentials; Animals; Animals, Newborn; Arrhythmias, Cardiac/genetics; Arrhythmias, Cardiac/pathology; Arrhythmias, Cardiac/physiopathology; Connexin 43/genetics; Connexin 43/physiology; Electrocardiography; Female; Heart Conduction System/physiopathology; Heart Ventricles/pathology; Heart Ventricles/physiopathology; Heterozygote; Male; Mice; Mice, Knockout; Phenotype

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CXA1

acts_upstream_of_or_within

GO:0061337: cardiac conduction

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857177

P

  • occurs_in:(EMAPA:17331)

Seeded From UniProt

complete

MOUSE:CXA1

acts_upstream_of_or_within

GO:0060373: regulation of ventricular cardiac muscle cell membrane depolarization

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857177

P

Seeded From UniProt

complete

MOUSE:CXA1

acts_upstream_of_or_within

GO:0061337: cardiac conduction

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857177

P

occurs_in:(EMAPA:17331)

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.