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PMID:9094723
Citation |
Hay, JC, Chao, DS, Kuo, CS and Scheller, RH (1997) Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells. Cell 89:149-58 |
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Abstract |
The proposed cis-Golgi vesicle receptor syntaxin 5 was found in a complex with Golgi-associated SNARE of 28 kDa (GOS-28), rbet1, rsly1, and two novel proteins characterized herein: rat sec22b and membrin, both cytoplasmically oriented integral membrane proteins. The complex appears to recapitulate vesicle docking interactions of proteins originating from distinct compartments, since syntaxin 5, rbet1, and GOS-28 localize to Golgi membranes, whereas mouse sec22b and membrin accumulate in the endoplasmic reticulum. Protein interactions in the complex are dramatically rearranged by N-ethylmaleimide-sensitive factor. The complex consists of two or more subcomplexes with some members (rat sec22b and syntaxin 5) in common and others (rbet1 and GOS-28) mutually exclusively associated. We propose that these protein interactions determine vesicle docking/fusion fidelity between the endoplasmic reticulum and Golgi. |
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Keywords |
Animals; Biological Transport/physiology; COS Cells/physiology; Carrier Proteins/physiology; Detergents; Endoplasmic Reticulum/chemistry; Endoplasmic Reticulum/metabolism; Epitopes/analysis; Gene Expression/physiology; Golgi Apparatus/chemistry; Golgi Apparatus/metabolism; Mammals; Membrane Proteins/analysis; Membrane Proteins/genetics; Membrane Proteins/isolation & purification; Membrane Proteins/physiology; Mice; Molecular Sequence Data; N-Ethylmaleimide-Sensitive Proteins; Nerve Tissue Proteins/analysis; Nerve Tissue Proteins/isolation & purification; Neurons/cytology; Neurons/metabolism; Qa-SNARE Proteins; Qb-SNARE Proteins; Qc-SNARE Proteins; R-SNARE Proteins; Rabbits; Rats; Rats, Sprague-Dawley; Sequence Homology, Amino Acid; Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; Subcellular Fractions/chemistry; Synaptic Vesicles/chemistry; Synaptic Vesicles/metabolism; Vesicular Transport Proteins |
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