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PMID:9092668
| Citation |
Shaw, LC and Lewin, AS (1997) The Cbp2 protein stimulates the splicing of the omega intron of yeast mitochondria. Nucleic Acids Res. 25:1597-604 |
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| Abstract |
The Cbp2 protein is encoded in the nucleus and is required for the splicing of the terminal intron of the mitochondrial COB gene in Saccharomyces cerevisiae . Using a yeast strain that lacks this intron but contains a related group I intron in the precursor of the large ribosomal RNA, we have determined that Cbp2 protein is also required for the normal accumulation of 21S ribosomal RNA in vivo . Such strains bearing a deletion of the CBP2 gene adapt slowly to growth in glycerol/ethanol media implying a defect in derepression. At physiologic concentrations of magnesium, Cbp2 stimulates the splicing of the ribosomal RNA intron in vitro . Nevertheless, Cbp2 is not essential for splicing of this intron in mitochondria nor is it required in vitro at magnesium concentrations >5 mM. A similar intron exists in the large ribosomal RNA (LSU) gene of Saccharomyces douglasii . This intron does need Cbp2 for catalytic activity in physiologic magnesium. Similarities between the LSU introns and COB intron 5 suggest that Cbp2 may recognize conserved elements of the these two introns, and protein-induced UV crosslinks occur in similar sites in the substrate and catalytic domains of the RNA precursors. |
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| Keywords |
Apoproteins/biosynthesis; Apoproteins/genetics; Base Sequence; Cell Nucleus/metabolism; Cytochrome b Group/biosynthesis; Cytochrome b Group/genetics; Cytochromes b; Fungal Proteins/metabolism; Genes, Fungal; Genotype; Introns; Kinetics; Magnesium/pharmacology; Mitochondria/metabolism; Molecular Sequence Data; Nucleic Acid Conformation; Oxygen Consumption; RNA Precursors/metabolism; RNA Splicing; RNA, Fungal/metabolism; RNA, Ribosomal/biosynthesis; Ribonucleoproteins; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; Sequence Deletion; Transcription, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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