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PMID:9043078

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Citation

Grant, B and Greenwald, I (1997) Structure, function, and expression of SEL-1, a negative regulator of LIN-12 and GLP-1 in C. elegans. Development 124:637-44

Abstract

Previous work indicated that sel-1 functions as a negative regulator of lin-12 activity, and predicted that SEL-1 is a secreted or membrane associated protein. In this study, we describe cell ablation experiments that suggest sel-1 mutations elevate lin-12 activity cell autonomously. We also use transgenic approaches to demonstrate that the predicted signal sequence of SEL-1 can direct secretion and is important for function, while a C-terminal hydrophobic region is not required for SEL-1 function. In addition, by analyzing SEL-1 localization using specific antisera we find that SEL-1 is localized intracellularly, with a punctate staining pattern suggestive of membrane bound vesicles. We incorporate these observations, and new information about a related yeast gene, into a proposal for a possible mechanism for SEL-1 function in LIN-12 turnover.

Links

PubMed

Keywords

Animals; Animals, Genetically Modified; Caenorhabditis elegans/genetics; Caenorhabditis elegans Proteins; Cell Lineage; Gene Expression Regulation, Developmental/genetics; Helminth Proteins/genetics; Membrane Glycoproteins/genetics; Membrane Proteins/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:G5EFG0

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:G5EFG0

involved_in

GO:0030433: ubiquitin-dependent ERAD pathway

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q05787

P

Seeded From UniProt

complete

CAEEL:G5EFG0

contributes_to

GO:0004842: ubiquitin-protein transferase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q05787

F

Seeded From UniProt

complete

CAEEL:G5EFG0

part_of

GO:0000836: Hrd1p ubiquitin ligase complex

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q05787

C

Seeded From UniProt

complete

See also

References

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