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PMID:9039828

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Citation

Savon, SP, Hakimi, P, Crawford, DR, Klemm, DJ, Gurney, AL and Hanson, RW (1997) The promoter regulatory regions of the genes for the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) from the chicken and the rat have different species-specific roles in gluconeogenesis. J. Nutr. 127:276-85

Abstract

Hepatic expression of the gene for phosphoenolpyruvate carboxykinase (GTP) (PEPCK-C) (EC 4.1.1.32) in birds occurs prior to birth and decreases to negligible levels before hatching, whereas in mammals the gene for PEPCK-C in the liver is expressed at birth and is active throughout the life of the animal. The administration of cyclic AMP to adult chickens results in the induction of transcription of the gene for PEPCK-C and the transient accumulation of PEPCK-C mRNA in the liver. DNase I footprint analysis of 330 bp of the avian PEPCK-C promoter immediately 5' of the start-site of transcription indicated the presence of several protein binding domains, purified CAAT/enhancer binding protein alpha, cAMP regulatory element binding protein and nuclear factor-1 bound to these regions of the promoter. Sequences corresponding to an hepatic nuclear factor-1 binding domain and to the insulin response sequence, previously identified in the rat PEPCK-C promoter, were also found in the chicken PEPCK-C promoter. Co-transfection of an expression vector for CAAT/enhancer binding protein alpha or CAAT/enhancer binding protein beta markedly stimulated transcription from both the chicken and rat PEPCK-C promoters in human hepatoma cells. Sequences involved in the regulation of gene transcription by cyclic AMP and insulin were found to reside between -210 and +1 of the avian PEPCK-C promoter. In general, transcription from the avian promoter was more sensitive to inhibition by insulin than was noted for the rat PEPCK-C promoter, which may explain in part the lack of expression of the gene for PEPCK-C in the livers of adult birds.

Links

PubMed Online version:10.1093/jn/127.2.276

Keywords

Animals; Base Sequence; Bucladesine/pharmacology; Chickens; Cytosol/enzymology; DNA/chemistry; Gluconeogenesis/genetics; Insulin/pharmacology; Liver/enzymology; Liver/ultrastructure; Male; Molecular Sequence Data; Phosphoenolpyruvate Carboxykinase (GTP)/genetics; Plasmids; Promoter Regions, Genetic/genetics; Promoter Regions, Genetic/physiology; RNA, Messenger/analysis; Rats; Rats, Sprague-Dawley; Species Specificity; Transcription, Genetic/drug effects; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:PCKGC

enables

GO:0050692: DNA binding domain binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:PCKGC

enables

GO:0004613: phosphoenolpyruvate carboxykinase (GTP) activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0006094: gluconeogenesis

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0006111: regulation of gluconeogenesis

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGM

enables

GO:0004613: phosphoenolpyruvate carboxykinase (GTP) activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHICK:PCKGM

involved_in

GO:0006094: gluconeogenesis

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGM

involved_in

GO:0006111: regulation of gluconeogenesis

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGM

involved_in

GO:0019516: lactate oxidation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGM

involved_in

GO:0019661: glucose catabolic process to lactate via pyruvate

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0032869: cellular response to insulin stimulus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0016042: lipid catabolic process

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0015743: malate transport

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

enables

GO:0043565: sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:PCKGC

enables

GO:0050692: DBD domain binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0071320: cellular response to cAMP

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0072071: kidney interstitial fibroblast differentiation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGC

enables

GO:0003729: mRNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:PCKGC

enables

GO:0019157: malate oxidase activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHICK:PCKGC

involved_in

GO:0006735: NADH regeneration

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGM

involved_in

GO:0072071: kidney interstitial fibroblast differentiation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGM

involved_in

GO:0070365: hepatocyte differentiation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGM

involved_in

GO:0016042: lipid catabolic process

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:PCKGM

involved_in

GO:0006735: NADH regeneration

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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