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PMID:9013326

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Citation

Hime, GR, Brill, JA and Fuller, MT (1996) Assembly of ring canals in the male germ line from structural components of the contractile ring. J. Cell. Sci. 109 ( Pt 12):2779-88

Abstract

Stable intercellular bridges called ring canals form following incomplete cytokinesis, and interconnect mitotically or meiotically related germ cells. We show that ring canals in Drosophila melanogaster males are surprisingly different from those previously described in females. Mature ring canal walls in males lack actin and appear to derive directly from structural proteins associated with the contractile ring. Ring canal assembly in males, as in females, initiates during cytokinesis with the appearance of a ring of phosphotyrosine epitopes at the site of the contractile ring. Following constriction, actin and myosin II disappear. However, at least four proteins present at the contractile ring remain: the three septins (Pnut, Sep1 and Sep2) and anillin. In sharp contrast, in ovarian ring canals, septins have not been detected, anillin is lost from mature ring canals and filamentous actin is a major component. In both males and females, a highly branched vesicular structure, termed the fusome, interconnects developing germ cells via the ring canals and is thought to coordinate mitotic germ cell divisions. We show that, in males, unlike females, the fusome persists and enlarges following cessation of the mitotic divisions, developing additional branches during meiosis. During differentiation, the fusome and its associated ring canals localize to the distal tip of the elongating spermatids.

Links

PubMed

Keywords

Actins/metabolism; Animals; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/metabolism; Contractile Proteins/metabolism; Drosophila melanogaster; Epitopes; Male; Meiosis; Models, Biological; Myosins/metabolism; Phosphoproteins/chemistry; Phosphorylation; Spermatocytes/chemistry; Spermatocytes/ultrastructure; Tyrosine/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:ANLN

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ANLN

located_in

GO:0045172: germline ring canal

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ANLN

involved_in

GO:0030726: male germline ring canal formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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