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PMID:9007251

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Citation

Ransom, DG, Haffter, P, Odenthal, J, Brownlie, A, Vogelsang, E, Kelsh, RN, Brand, M, van Eeden, FJ, Furutani-Seiki, M, Granato, M, Hammerschmidt, M, Heisenberg, CP, Jiang, YJ, Kane, DA, Mullins, MC and Nüsslein-Volhard, C (1996) Characterization of zebrafish mutants with defects in embryonic hematopoiesis. Development 123:311-9

Abstract

As part of a large scale chemical mutagenesis screen of the zebrafish (Danio rerio) genome, we have identified 33 mutants with defects in hematopoiesis. Complementation analysis placed 32 of these mutants into 17 complementation groups. The allelism of the remaining 1 blood mutant is currently unresolved. We have categorized these blood mutants into four phenotypic classes based on analyses of whole embryos and isolated blood cells, as well as by in situ hybridization using the hematopoietic transcription factors GATA-1 and GATA-2. Embryos mutant for the gene moonshine have few if any proerythroblasts visible on the day circulation begins and normal erythroid cell differentiation is blocked as determined by staining for hemoglobin and GATA-1 expression. Mutations in five genes, chablis, frascati, merlot, retsina, thunderbird and two possibly unique mutations cause a progressive decrease in the number of blood cells during the first 5 days of development. Mutations in another seven genes, chardonnay, chianti, grenache, sauternes, weiflherbst and zinfandel, and two additional mutations result in hypochromic blood cells which also decrease in number as development proceeds. Several of these mutants have immature cells in the circulation, indicating a block in normal erythroid development. The mutation in zinfandel is dominant, and 2-day old heterozygous carriers fail to express detectable levels of hemoglobin and have decreasing numbers of circulating cells during the first 5 days of development. Mutations in two genes, freixenet and yquem, result in the animals that are photosensitive with autofluorescent blood, similar to that found in the human congenital porphyrias. The collection of mutants presented here represent several steps required for normal erythropoiesis. The analysis of these mutants provides a powerful approach towards defining the molecular mechanisms involved in vertebrate hematopoietic development.

Links

PubMed

Keywords

Anemia, Hypochromic/blood; Anemia, Hypochromic/embryology; Anemia, Hypochromic/genetics; Animals; Embryo, Nonmammalian/blood supply; Erythrocyte Count; Hematopoiesis/genetics; Hemoglobins/metabolism; Light/adverse effects; Mutation; Phenotype; Zebrafish/blood; Zebrafish/embryology; Zebrafish/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:A0A2R9YJH2

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1080

P

Seeded From UniProt

complete

DANRE:A0A2R9YJH2

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-101220-3

P

Seeded From UniProt

complete

DANRE:A4QN38

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1080

P

Seeded From UniProt

complete

DANRE:A4QN38

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-101220-3

P

Seeded From UniProt

complete

DANRE:A7MD95

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1080

P

Seeded From UniProt

complete

DANRE:A7MD95

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-101220-3

P

Seeded From UniProt

complete

DANRE:B3DGS9

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-517

P

Seeded From UniProt

complete

DANRE:B3DGY6

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-001229-2

P

Seeded From UniProt

complete

DANRE:B3DHD1

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-862

P

Seeded From UniProt

complete

DANRE:B3DKQ1

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1080

P

Seeded From UniProt

complete

DANRE:B3DKQ1

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-101220-3

P

Seeded From UniProt

complete

DANRE:F1QKX3

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-517

P

Seeded From UniProt

complete

DANRE:F1QQE5

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-001229-2

P

Seeded From UniProt

complete

DANRE:Q08CS0

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1080

P

Seeded From UniProt

complete

DANRE:Q08CS0

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-101220-3

P

Seeded From UniProt

complete

DANRE:Q1RLY4

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-517

P

Seeded From UniProt

complete

DANRE:MFRN1

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-532

P

Seeded From UniProt

complete

DANRE:MFRN1

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-518

P

Seeded From UniProt

complete

DANRE:MFRN1

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-274

P

Seeded From UniProt

complete

DANRE:MFRN1

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1256

P

Seeded From UniProt

complete

DANRE:Q567M0

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1080

P

Seeded From UniProt

complete

DANRE:Q567M0

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-101220-3

P

Seeded From UniProt

complete

DANRE:Q5MPX4

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-862

P

Seeded From UniProt

complete

DANRE:TRI33

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-974

P

Seeded From UniProt

complete

DANRE:TRI33

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1309

P

Seeded From UniProt

complete

DANRE:TRI33

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1308

P

Seeded From UniProt

complete

DANRE:TRI33

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1306

P

Seeded From UniProt

complete

DANRE:TRI33

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1305

P

Seeded From UniProt

complete

DANRE:TRI33

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1304

P

Seeded From UniProt

complete

DANRE:TRI33

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1303

P

Seeded From UniProt

complete

DANRE:TRI33

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1302

P

Seeded From UniProt

complete

DANRE:Q7ZZJ7

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1080

P

Seeded From UniProt

complete

DANRE:Q7ZZJ7

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-101220-3

P

Seeded From UniProt

complete

DANRE:Q7ZZV6

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1080

P

Seeded From UniProt

complete

DANRE:Q7ZZV6

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-101220-3

P

Seeded From UniProt

complete

DANRE:Q8AX90

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-517

P

Seeded From UniProt

complete

DANRE:Q8JFT9

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1080

P

Seeded From UniProt

complete

DANRE:Q8JFT9

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-101220-3

P

Seeded From UniProt

complete

DANRE:Q8JG61

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-69

P

Seeded From UniProt

complete

DANRE:Q8JG61

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1287

P

Seeded From UniProt

complete

DANRE:Q9DGM6

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-001229-2

P

Seeded From UniProt

complete

DANRE:S40A1

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-94

P

Seeded From UniProt

complete

DANRE:S40A1

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-802

P

Seeded From UniProt

complete

DANRE:Q9PUF1

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-517

P

Seeded From UniProt

complete

DANRE:HEM0

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1405

P

Seeded From UniProt

complete

DANRE:HEM0

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1025

P

Seeded From UniProt

complete

See also

References

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