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PMID:8987804

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Citation

Landsend, AS, Amiry-Moghaddam, M, Matsubara, A, Bergersen, L, Usami, S, Wenthold, RJ and Ottersen, OP (1997) Differential localization of delta glutamate receptors in the rat cerebellum: coexpression with AMPA receptors in parallel fiber-spine synapses and absence from climbing fiber-spine synapses. J. Neurosci. 17:834-42

Abstract

The delta 2 glutamate receptors are prominently expressed in Purkinje cells and are thought to play a key role in the induction of cerebellar long-term depression. The synaptic and subsynaptic localization of delta receptors in rat cerebellar cortex was investigated with sensitive and high-resolution immunogold procedures. After postembedding incubation with an antibody raised to a C-terminal peptide of delta 2, high gold particle densities occurred in all parallel fiber synapses with Purkinje cell dendritic spines, whereas other synapses were consistently devoid of labeling. Among the types of immunonegative synapse were climbing fiber synapses with spines and parallel fiber synapses with dendritic stems of interneurons. At the parallel fiber-spine synapse, gold particles signaling delta receptors were restricted to the postsynaptic specialization. By the use of double labeling with two different gold particle sizes, it was shown that delta and AMPA GluR2/3 receptors were colocalized along the entire extent of the postsynaptic specialization without forming separate domains. The distribution of gold particles representing delta receptors was consistent with a cytoplasmic localization of the C terminus and an absence of a significant presynaptic pool of receptor molecules. The present data suggest that the delta 2 receptors are targeted selectively to a subset of Purkinje cell spines and that they are coexpressed with ionotropic receptors in the postsynaptic specialization. This arrangement could allow for a direct interaction between the two classes of receptor.

Links

PubMed

Keywords

Animals; Cerebellum/chemistry; Cerebellum/ultrastructure; Dendrites/chemistry; Immunohistochemistry; Male; Nerve Fibers/chemistry; Nerve Tissue Proteins/analysis; Nerve Tissue Proteins/biosynthesis; Purkinje Cells/chemistry; Rats; Rats, Wistar; Receptors, AMPA/analysis; Receptors, AMPA/biosynthesis; Receptors, Glutamate/analysis; Receptors, Glutamate/biosynthesis; Synapses/chemistry

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:GRID1

located_in

GO:0045211: postsynaptic membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:GRID2

located_in

GO:0045211: postsynaptic membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:GRID2

located_in

GO:0099061: integral component of postsynaptic density membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:GRID2

located_in

GO:0099061: integral component of postsynaptic density membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:GRID2

located_in

GO:0098978: glutamatergic synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0002037)

Seeded From UniProt

complete

RAT:GRID2

located_in

GO:0098688: parallel fiber to Purkinje cell synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:GRID2

part_of

GO:0045211: postsynaptic membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:GRID2

part_of

GO:0098688: parallel fiber to Purkinje cell synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:GRID2

part_of

GO:0098978: glutamatergic synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

part_of:(UBERON:0002037)

Seeded From UniProt

complete

RAT:GRID2

part_of

GO:0099061: integral component of postsynaptic density membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

part_of:(GO:0098688)

Seeded From UniProt

complete

RAT:GRID2

part_of

GO:0099061: integral component of postsynaptic density membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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