GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:8978043

From GONUTS
Jump to: navigation, search
Citation

Schnorr, JD and Berg, CA (1996) Differential activity of Ras1 during patterning of the Drosophila dorsoventral axis. Genetics 144:1545-57

Abstract

In Drosophila, the Ras1 gene is required downstream of receptor tyrosine kinases for correct eye development, embryonic patterning, wing vein formation, and border cell migration. Here we characterize a P-element allele of Ras1, Ras1(5703), that affects viability, eye morphogenesis, and early and late stages of oogenesis. Flies transheterozgyous for Ras1(5703) and existing EMS-induced Ras1 alleles are viable and exhibit a range of eye and eggshell defects. Differences in the severity of these phenotypes in different tissues suggest that there are allele-specific effects of Ras1 in development. Analysis of rescue constructs demonstrates that these differential phenotypes are due to loss of function in Ras1 alone and not due to effects on neighboring genes. Females mutant at the Ras1 locus lay eggs with reduced or missing dorsal eggshell structures. We observe dominant interactions between Ras1 mutants and other dorsoventral pathway mutants, including Egfr(top) and gurken. Ras1 is also epistatic to K10. Unlike Egfr(top) and gurken mutants, however, Ras1 females are moderately fertile, laying eggs with ventralized eggshells that can hatch normal larvae. These results suggest that Ras1 may have a different requirement in the patterning of the eggshell axis than in the patterning of the embryonic axis during oogenesis.

Links

PubMed PMC1207707

Keywords

Alleles; Animals; Base Sequence; Chromosome Mapping; DNA Transposable Elements/genetics; Drosophila/embryology; Drosophila/genetics; Drosophila/metabolism; Gene Expression Regulation, Developmental; Genes, ras; Molecular Sequence Data; ras Proteins/genetics; ras Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:RAS1

involved_in

GO:0007173: epidermal growth factor receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0001137

P

Seeded From UniProt

complete

DROME:RAS1

involved_in

GO:0007173: epidermal growth factor receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0003731

P

Seeded From UniProt

complete

DROME:EGFR

involved_in

GO:0007173: epidermal growth factor receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0003205

P

Seeded From UniProt

complete

DROME:GRK

involved_in

GO:0007173: epidermal growth factor receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0003205

P

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.