GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:8970152

From GONUTS
Jump to: navigation, search
Citation

Podbilewicz, B (1996) ADM-1, a protein with metalloprotease- and disintegrin-like domains, is expressed in syncytial organs, sperm, and sheath cells of sensory organs in Caenorhabditis elegans. Mol. Biol. Cell 7:1877-93

Abstract

A search was carried out for homologues of possible fusogenic proteins to study their function in a genetically tractable animal. The isolation, molecular, and cellular characterization of the Caenorhabditis elegans adm-1 gene (a disintegrin and metalloprotease domain) are described. A glycoprotein analogous to viral fusion proteins has been identified on the surface of guinea pig sperm (PH-30/fertilin) and is implicated in sperm-egg fusion. adm-1 is the first reported invertebrate gene related to PH-30 and a family of proteins containing snake venom disintegrin- and metalloprotease-like domains. ADM-1 shows a domain organization identical to PH-30. It contains prepro, metalloprotease, disintegrin, cysteine rich with putative fusion peptide, epidermal growth factor-like repeat, transmembrane, and cytoplasmic domains. Antibodies which recognize ADM-1 protein in immunoblots were generated. Using immunofluorescence and in situ hybridization, the products of adm-1 have been detected in specific cells during different stages of development. The localization of ADM-1 to the plasma membrane of embryonic cells and to the sheath cells of sensory organs suggests a function in cell adhesion. ADM-1 expression in the hypodermis, pharynx, vulva, and mature sperm is consistent with a putative role in somatic and gamete cell fusions.

Links

PubMed PMC276037

Keywords

ADAM Proteins; Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins; Chromosome Mapping; DNA, Complementary; Disintegrins/chemistry; Disintegrins/genetics; Disintegrins/metabolism; Helminth Proteins/genetics; Helminth Proteins/metabolism; Male; Membrane Glycoproteins/chemistry; Metalloendopeptidases/chemistry; Metalloendopeptidases/genetics; Metalloendopeptidases/metabolism; Molecular Sequence Data; RNA, Messenger; Sequence Homology, Amino Acid; Spermatozoa/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:UNC71

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:UNC71

involved_in

GO:0006508: proteolysis

ECO:0000250: sequence similarity evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:UNC71

enables

GO:0008237: metallopeptidase activity

ECO:0000250: sequence similarity evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:UNC71

involved_in

GO:0098609: cell-cell adhesion

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CAEEL:UNC71

involved_in

GO:0045026: plasma membrane fusion

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

See also

References

See Help:References for how to manage references in GONUTS.