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PMID:8929543

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Citation

Baumann, P, Benson, FE and West, SC (1996) Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro. Cell 87:757-66

Abstract

The human testis Rad51 protein, a structural homolog of E. coli RecA, binds single- and double-stranded DNA and exhibits DNA-dependent ATPase activity. Using circular ssDNA and linear dsDNA (3.0 kb in length), we demonstrate that hRad51 promotes homologous pairing and strand exchange reactions in vitro. Joint molecule formation was dependent upon ATP hydrolysis and DNA homology and was stimulated by the single-strand DNA-binding protein RP-A. In these reactions, the 5' terminus of the complementary strand of the linear duplex was efficiently transferred to the ssDNA. However, under standard conditions, extensive strand exchange was not observed. These results establish hRad51 as a functional homolog of RecA, but indicate that the human protein and its bacterial counterpart differ in their ability to promote extensive strand transfer. It is proposed that hRad51 mediates homology recognition and initiates strand exchange, but that extensive heteroduplex formation in higher organisms requires the actions of additional proteins.

Links

PubMed

Keywords

Adenosine Triphosphate/metabolism; Chemical Precipitation; DNA/metabolism; DNA Ligases/metabolism; DNA, Circular; DNA, Single-Stranded/metabolism; DNA-Binding Proteins/metabolism; Humans; Male; Nucleic Acid Conformation; Nucleoproteins/metabolism; Protein Binding; Rad51 Recombinase; Rec A Recombinases/genetics; Recombination, Genetic; Sequence Homology, Nucleic Acid; Species Specificity; Spermidine; Testis/chemistry

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:RAD51

enables

GO:0017116: single-stranded DNA helicase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:RAD51

involved_in

GO:0051106: positive regulation of DNA ligation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:RAD51

enables

GO:0043142: single-stranded DNA-dependent ATPase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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