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PMID:8900209

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Citation

Zimmermann, G and Taussig, R (1996) Protein kinase C alters the responsiveness of adenylyl cyclases to G protein alpha and betagamma subunits. J. Biol. Chem. 271:27161-6

Abstract

The ability of protein kinase C (PKC) to regulate the responsiveness of adenylyl cyclase to different activators was assessed. Membranes prepared from Sf9 cells infected with recombinant baculoviruses encoding either type II or IV adenylyl cyclase were incubated with recombinant PKCalpha (purified from Sf9 cells), and the effects on adenylyl cyclase activity were measured after reconstitution with Gsalpha, Gbetagamma, or forskolin. PKCalpha treatment of type II adenylyl cyclase leads to increases in basal, forskolin-stimulated, and betagamma-stimulated activities and greater sensitivity to stimulation by Gsalpha. Paradoxically, most of the betagamma potentiation of Gsalpha-stimulated activity is eliminated by pretreatment with PKCalpha. By contrast, treatment of type IV adenylyl cyclase with PKCalpha has little effect on the basal, forskolin-stimulated, or betagamma-stimulated activities but markedly reduces the Gsalpha-stimulated and betagamma-potentiated activity of this isoform. These studies demonstrate that protein kinases can alter both the activity of adenylyl cyclase isoforms and their responsiveness to G protein regulation, thereby altering the ability of adenylyl cyclases to integrate signals derived from multiple hormonal inputs.

Links

PubMed

Keywords

Adenylate Cyclase/metabolism; Animals; Baculoviridae/genetics; Cell Line; GTP-Binding Proteins/metabolism; Protein Kinase C/genetics; Protein Kinase C/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Spodoptera

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BOVIN:G0TC75

involved_in

GO:0006171: cAMP biosynthetic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BOVIN:G0TC75

enables

GO:0004016: adenylate cyclase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

BOVIN:G0TC75

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:ADCY4

involved_in

GO:0006171: cAMP biosynthetic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:ADCY4

enables

GO:0004016: adenylate cyclase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

RAT:ADCY4

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BOVIN:KPCA

enables

GO:0004697: protein kinase C activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

BOVIN:KPCA

involved_in

GO:0006468: protein phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BOVIN:KPCA

involved_in

GO:0106071: positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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