PMID:8900209

Citation	Zimmermann, G and Taussig, R (1996) Protein kinase C alters the responsiveness of adenylyl cyclases to G protein alpha and betagamma subunits. J. Biol. Chem. 271:27161-6
Abstract	The ability of protein kinase C (PKC) to regulate the responsiveness of adenylyl cyclase to different activators was assessed. Membranes prepared from Sf9 cells infected with recombinant baculoviruses encoding either type II or IV adenylyl cyclase were incubated with recombinant PKCalpha (purified from Sf9 cells), and the effects on adenylyl cyclase activity were measured after reconstitution with Gsalpha, Gbetagamma, or forskolin. PKCalpha treatment of type II adenylyl cyclase leads to increases in basal, forskolin-stimulated, and betagamma-stimulated activities and greater sensitivity to stimulation by Gsalpha. Paradoxically, most of the betagamma potentiation of Gsalpha-stimulated activity is eliminated by pretreatment with PKCalpha. By contrast, treatment of type IV adenylyl cyclase with PKCalpha has little effect on the basal, forskolin-stimulated, or betagamma-stimulated activities but markedly reduces the Gsalpha-stimulated and betagamma-potentiated activity of this isoform. These studies demonstrate that protein kinases can alter both the activity of adenylyl cyclase isoforms and their responsiveness to G protein regulation, thereby altering the ability of adenylyl cyclases to integrate signals derived from multiple hormonal inputs.
Links	PubMed
Keywords	Adenylate Cyclase/metabolism; Animals; Baculoviridae/genetics; Cell Line; GTP-Binding Proteins/metabolism; Protein Kinase C/genetics; Protein Kinase C/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Spodoptera
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BOVIN:G0TC75	involved_in	GO:0006171: cAMP biosynthetic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
BOVIN:G0TC75	enables	GO:0004016: adenylate cyclase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
BOVIN:G0TC75	located_in	GO:0016020: membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:ADCY4	involved_in	GO:0006171: cAMP biosynthetic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
RAT:ADCY4	enables	GO:0004016: adenylate cyclase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
RAT:ADCY4	located_in	GO:0016020: membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
BOVIN:KPCA	enables	GO:0004697: protein kinase C activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
BOVIN:KPCA	involved_in	GO:0006468: protein phosphorylation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
BOVIN:KPCA	involved_in	GO:0106071: positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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