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PMID:8861941

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Citation

Robb, L, Elwood, NJ, Elefanty, AG, Köntgen, F, Li, R, Barnett, LD and Begley, CG (1996) The scl gene product is required for the generation of all hematopoietic lineages in the adult mouse. EMBO J. 15:4123-9

Abstract

Homozygosity for a null mutation in the scl gene causes mid-gestational embryonic lethality in the mouse due to failure of development of primitive hematopoiesis. Whilst this observation established the role of the scl gene product in primitive hematopoiesis, the death of the scl null embryos precluded analysis of the role of scl in later hematopoietic development. To address this question, we created embryonic stem cell lines with a homozygous null mutation of the scl gene (scl-/-) and used these lines to derive chimeric mice. Analysis of the chimeric mice demonstrates that the scl-/- embryonic stem cells make a substantial contribution to all non-hematopoietic tissues but do not contribute to any hematopoietic lineage. These observations reveal a crucial role for the scl gene product in definitive hematopoiesis. In addition, in vitro differentiation assays with scl-/- embryonic stem cells showed that the scl gene product was also required for formation of hematopoietic cells in this system.

Links

PubMed PMC452135

Keywords

Animals; Basic Helix-Loop-Helix Transcription Factors; Bone Marrow Cells; Cell Lineage; Chimera/genetics; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Female; Gene Targeting; Genes, Lethal; Genotype; Hematopoiesis/genetics; Liver/cytology; Liver/embryology; Mice/embryology; Mice/genetics; Mice/physiology; Mice, Inbred C57BL; Mice, Knockout; Organ Specificity; Proto-Oncogene Proteins; Stem Cells; Transcription Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:TAL1

acts_upstream_of_or_within

GO:0060216: definitive hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857566

P

Seeded From UniProt

complete


See also

References

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