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PMID:8861722

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Citation

Staecker, H, Van De Water, TR, Lefebvre, PP, Liu, W, Moghadassi, M, Galinovic-Schwartz, V, Malgrange, B and Moonen, G (1996) NGF, BDNF and NT-3 play unique roles in the in vitro development and patterning of innervation of the mammalian inner ear. Brain Res. Dev. Brain Res. 92:49-60

Abstract

Developing cochleovestibular ganglion (CVG) neurons depend upon interaction with the otocyst, their peripheral target tissue, for both trophic support and tropic guidance. RT-PCR of E11 through E14 otocyst-CVG RNA extracts have shown that NGF as well as BDNF and NT-3 are expressed in the developing inner ear (in situ RT-PCR on tissue sections of E12 otocysts localized all three neurotrophins to the otocyst). To evaluate the functional significance of NGF, BDNF and NT-3 expression, E10.5 otocyst-CVG explants were treated with antisense oligonucleotides and compared to sense treated and control cultures. Confocal microscopic analysis revealed that treatment with BDNF antisense resulted in extensive neuronal cell death, downregulation of NGF caused an inhibition of neuritogenesis and a decrease in the neuronal population of the CVG, whereas treatment with NT-3 antisense resulted in a loss of target directed CVG neuritic ingrowth in this in vitro model. The effect of NGF or BDNF antisense treatment could be prevented by the simultaneous addition of the respective growth factor. These findings demonstrate that each of the three neurotrophins have important roles during the onset of neuritic ingrowth of the CVG neurons to the otocyst.

Links

PubMed

Keywords

Animals; Antisense Elements (Genetics)/pharmacology; Brain-Derived Neurotrophic Factor/genetics; Brain-Derived Neurotrophic Factor/physiology; Cell Survival/drug effects; Ear, Inner/embryology; Embryo, Mammalian/physiology; Embryonic and Fetal Development; Mice/embryology; Mice, Inbred Strains; Nerve Growth Factors/genetics; Nerve Growth Factors/physiology; Neural Pathways/embryology; Neurites/physiology; Neurons/drug effects; Neurotensin/genetics; Neurotensin/physiology; RNA, Messenger/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:NGF

acts_upstream_of_or_within

GO:0045773: positive regulation of axon extension

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • regulates_o_occurs_in:(CL:0000540)
  • regulates_o_occurs_in:(EMAPA:17571)

Seeded From UniProt

complete

MOUSE:NTF3

acts_upstream_of_or_within

GO:0007411: axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(EMAPA:17571)

Seeded From UniProt

complete

MOUSE:BDNF

acts_upstream_of_or_within

GO:0043524: negative regulation of neuron apoptotic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(EMAPA:17571)
  • regulates_o_occurs_in:(CL:0000540)

Seeded From UniProt

complete

See also

References

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