GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:8824590
Citation |
Chin-Sang, ID and Spence, AM (1996) Caenorhabditis elegans sex-determining protein FEM-2 is a protein phosphatase that promotes male development and interacts directly with FEM-3. Genes Dev. 10:2314-25 |
---|---|
Abstract |
Male sexual development in the nematode Caenorhabditis elegans requires the genes fem-1, fem-2, and fem-3. The current model of sex determination portrays the FEM proteins as components of a novel signal transduction pathway, but the mechanisms involved in signaling through the pathway are not understood. We report the isolation of fem-2 cDNAs in a yeast two-hybrid screen for clones encoding proteins that interact with FEM-3. Association of FEM-3 and FEM-2 in two independent in vitro binding assays substantiates the interaction detected in the two-hybrid system. FEM-2 is related in sequence to protein serine/threonine phosphatases of Type 2C (PP2C). We demonstrate that FEM-2 exhibits magnesium-dependent casein phosphatase activity, typical of PP2C, in vitro. Point mutations that abolish the casein phosphatase activity of FEM-2 without affecting its FEM-3-binding activity reduce severely its ability to rescue male development in fem-2 mutant nematodes. These results suggest that protein phosphorylation regulates sex determination in C. elegans. |
Links | |
Keywords |
Amino Acid Sequence; Animals; Caenorhabditis elegans/enzymology; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins; Enzyme Activation/genetics; Helminth Proteins/chemistry; Helminth Proteins/genetics; Helminth Proteins/metabolism; Hybrid Cells; Male; Molecular Sequence Data; Mutation; Phosphoprotein Phosphatases/chemistry; Phosphoprotein Phosphatases/metabolism; Protein Phosphatase 2; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Sex Differentiation/genetics; Yeasts/genetics |
edit table |
Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
CAEEL:H2L280 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
WB:WBGene00001413 |
F |
Seeded From UniProt |
complete | |
CAEEL:FEM1 |
involved_in |
GO:0007530: sex determination |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
CAEEL:FEM3 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
WB:WBGene00001413 |
F |
Seeded From UniProt |
complete | |
CAEEL:FEM3 |
involved_in |
GO:0007530: sex determination |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
CAEEL:FEM2 |
enables |
GO:0004722: protein serine/threonine phosphatase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
CAEEL:FEM2 |
involved_in |
GO:0006470: protein dephosphorylation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
CAEEL:FEM2 |
involved_in |
GO:0045138: nematode male tail tip morphogenesis |
ECO:0000315: mutant phenotype evidence used in manual assertion |
WB:WBVar00144567 |
P |
Seeded From UniProt |
complete | |
CAEEL:FEM2 |
involved_in |
GO:0030238: male sex determination |
ECO:0000315: mutant phenotype evidence used in manual assertion |
WB:WBVar00144567 |
P |
Seeded From UniProt |
complete | |
CAEEL:FEM2 |
involved_in |
GO:0042006: masculinization of hermaphroditic germ-line |
ECO:0000315: mutant phenotype evidence used in manual assertion |
WB:WBVar00144567 |
P |
Seeded From UniProt |
complete | |
CAEEL:FEM2 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
WB:WBGene00001413 |
F |
Seeded From UniProt |
complete | |
CAEEL:FEM2 |
involved_in |
GO:0007530: sex determination |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
See also
References
See Help:References for how to manage references in GONUTS.