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PMID:8752211

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Citation

Thuret, JY, Valay, JG, Faye, G and Mann, C (1996) Civ1 (CAK in vivo), a novel Cdk-activating kinase. Cell 86:565-76

Abstract

Cyclin-dependent protein kinases (Cdks) play key roles in regulating cell division and gene expression. Most Cdks require binding of a cyclin and phosphorylation by a Cdk-activating kinase (CAK) to be active. We report the identification of Civ1 (CAK in vivo), a novel CAK activity in S. cerevisiae. Civ1 is most similar in sequence to the Cdks, but unlike them is active as a monomer and may thus be the founding member of a novel family of kinases. Civ1 binds tightly to and phosphorylates Cdc28, thereby allowing its subsequent activation by the binding of a cyclin. The CIV1 gene is essential for yeast cell viability, and Cdc28 phosphorylation and activity are conditionally inhibited in a civ1-4 temperature-sensitive mutant. Civ1 is the only CAK for which there are genetic data indicating that its activity is physiologically relevant in vivo.

Links

PubMed

Keywords

Amino Acid Sequence; Base Sequence; CDC28 Protein Kinase, S cerevisiae/metabolism; Cell Cycle; Cell Cycle Proteins/genetics; Cyclin-Dependent Kinases; DNA Primers/chemistry; Fungal Proteins/genetics; Genes, Fungal; Molecular Sequence Data; Phosphorylation; Protein-Serine-Threonine Kinases/genetics; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Sequence Alignment; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:CAK1

enables

GO:0019912: cyclin-dependent protein kinase activating kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:CAK1

enables

GO:0019912: cyclin-dependent protein kinase activating kinase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:CAK1

involved_in

GO:0000086: G2/M transition of mitotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:CAK1

involved_in

GO:0006468: protein phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:CAK1

involved_in

GO:0006468: protein phosphorylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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