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PMID:8702761

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Citation

Arselin, G, Vaillier, J, Graves, PV and Velours, J (1996) ATP synthase of yeast mitochondria. Isolation of the subunit h and disruption of the ATP14 gene. J. Biol. Chem. 271:20284-90

Abstract

A new subunit of the yeast ATP synthase (termed subunit h) has been isolated. Amino acid composition and N-terminal sequencing were determined by chemical methods. These data were in agreement with the sequence of the hypothetical protein L8003.20 whose primary structure was deduced from DNA sequencing of the yeast chromosome XII. The amino acid sequence encoded by ATP14 gene is 32 amino acids longer than the mature protein, which contains 92 amino acids corresponding to a calculated mass of 10,408 Da. The protein is hydrophilic and acidic with a calculated pHi of 4.08. It is not apparently related to any subunit described in other ATP synthases. A null mutant was constructed. The mutation was recessive and the mutant strain was unable to grow on glycerol medium. A high percentage of rho- cells arose spontaneously. The mutant mitochondria had no detectable oligomycin-sensitive ATPase activity, but still contained ATPase activity with a catalytic sector dissociated from the membranous components. The mutant mitochondria did not contain subunit h, and the mitochondrially encoded hydrophobic subunit 6 was not present.

Links

PubMed

Keywords

Amino Acid Sequence; Base Sequence; Energy Metabolism; Genes, Fungal; Macromolecular Substances; Mitochondria/enzymology; Mitochondrial Proton-Translocating ATPases; Molecular Sequence Data; Mutagenesis, Insertional; Proton-Translocating ATPases/chemistry; Proton-Translocating ATPases/genetics; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:ATP14

part_of

GO:0000276: mitochondrial proton-transporting ATP synthase complex, coupling factor F(o)

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:ATP14

involved_in

GO:0015986: proton motive force-driven ATP synthesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:ATP14

contributes_to

GO:0046933: proton-transporting ATP synthase activity, rotational mechanism

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

See also

References

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