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PMID:8663349

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Citation

Ye, Q and Worman, HJ (1996) Interaction between an integral protein of the nuclear envelope inner membrane and human chromodomain proteins homologous to Drosophila HP1. J. Biol. Chem. 271:14653-6

Abstract

At the nuclear envelope in higher eukaryotic cells, the nuclear lamina and the heterochromatin are adjacent to the inner nuclear membrane, and their attachment is presumably mediated by integral membrane proteins. In a yeast two-hybrid screen, the nucleoplasmic domain of lamin B receptor (LBR), an integral protein of the inner nuclear membrane, associated with two human polypeptides homologous to Drosophila HP1, a heterochromatin protein involved in position-effect variegation. LBR fusion proteins bound to HP1 proteins synthesized by in vitro translation and present in cell lysates. Antibodies against LBR also co-immunoprecipitated HP1 proteins from cell extracts. LBR can interact with chromodomain proteins that are highly conserved in eukaryotic species and may function in the attachment of heterochromatin to the inner nuclear membrane in cells.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Binding Sites; Chromosomal Proteins, Non-Histone/biosynthesis; Chromosomal Proteins, Non-Histone/chemistry; Chromosomal Proteins, Non-Histone/metabolism; Cloning, Molecular; Conserved Sequence; Drosophila melanogaster/metabolism; Humans; Molecular Sequence Data; Nuclear Envelope/metabolism; Receptors, Cytoplasmic and Nuclear/metabolism; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:CBX5

located_in

GO:0000792: heterochromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CBX5

located_in

GO:0005635: nuclear envelope

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CBX5

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q14739

F

Seeded From UniProt

complete

HUMAN:CBX3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q14739

F

Seeded From UniProt

complete

HUMAN:CBX3

located_in

GO:0005637: nuclear inner membrane

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:LBR

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P45973

F

Seeded From UniProt

complete

HUMAN:LBR

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q13185

F

Seeded From UniProt

complete

HUMAN:CBX5

part_of

GO:0005720: nuclear heterochromatin

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CBX5

part_of

GO:0005635: nuclear envelope

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CBX3

part_of

GO:0005637: nuclear inner membrane

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CBX3

involved_in

GO:0006338: chromatin remodeling

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CBX3

enables

GO:0019904: protein domain specific binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q14739

F

Seeded From UniProt

complete


See also

References

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