PMID:8663286

Citation	Si, K, Das, K and Maitra, U (1996) Characterization of multiple mRNAs that encode mammalian translation initiation factor 5 (eIF-5). J. Biol. Chem. 271:16934-8
Abstract	Eukaryotic translation initiation factor 5 (eIF-5) interacts with the 40 S initiation complex (40S.mRNA.MettRNAf.eIF-2.GTP) to promote the hydrolysis of bound GTP with the concomitant joining of the 60 S ribosomal subunit to the 40 S initiation complex to form a functional 80 S initiation complex. In this paper, the multiple mRNAs that encode mammalian eIF-5 have been characterized. In rat tissues, three major eIF-5 mRNAs of 3.5, 2.8, and 2.2 kilobases in length are detected. All major eIF-5 mRNAs are initiated from a single transcription initiation site, contain identical 5'-untranslated and coding regions, but differ from one another only in the length of their 3'-untranslated regions. The different lengths of the 3'-untranslated region of eIF-5 mRNAs are generated by the use of alternative polyadenylation signals. Additionally, we demonstrate tissue-specific variations in eIF-5 mRNA expression as well as preference for polyadenylation sites. These results should lead to increased understanding of the regulation of eIF-5 gene expression.
Links	PubMed
Keywords	Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA, Complementary; Eukaryotic Initiation Factor-5; HeLa Cells; Humans; Molecular Sequence Data; Open Reading Frames; Peptide Initiation Factors/genetics; Poly A/metabolism; RNA, Messenger/genetics; Rats; Saccharomyces cerevisiae/genetics; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:IF5	enables	GO:0003924: GTPase activity	ECO:0000304: author statement supported by traceable reference used in manual assertion		F		Seeded From UniProt	complete
HUMAN:IF5	part_of	GO:0005737: cytoplasm	ECO:0000304: author statement supported by traceable reference used in manual assertion		C		Seeded From UniProt	complete
HUMAN:IF5	involved_in	GO:0006446: regulation of translational initiation	ECO:0000304: author statement supported by traceable reference used in manual assertion		P		Seeded From UniProt	complete
HUMAN:IF5	enables	GO:0008135: translation factor activity, RNA binding	ECO:0000304: author statement supported by traceable reference used in manual assertion		F		Seeded From UniProt	complete
HUMAN:IF5	enables	GO:0003743: translation initiation factor activity	ECO:0000303: author statement without traceable support used in manual assertion		F		Seeded From UniProt	complete
HUMAN:IF5	part_of	GO:0005829: cytosol	ECO:0000303: author statement without traceable support used in manual assertion		C		Seeded From UniProt	complete
HUMAN:IF5	involved_in	GO:0006413: translational initiation	ECO:0000303: author statement without traceable support used in manual assertion		P		Seeded From UniProt	complete

References

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