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PMID:8643484

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Citation

Dreyling, MH, Martinez-Climent, JA, Zheng, M, Mao, J, Rowley, JD and Bohlander, SK (1996) The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family. Proc. Natl. Acad. Sci. U.S.A. 93:4804-9

Abstract

The translocation t(10;11)(p13;q14) is a recurring chromosomal abnormality that has been observed in patients with acute lymphoblastic leukemia as well as acute myeloid leukemia. We have recently reported that the monocytic cell line U937 has a t(10;11)(p13;q14) translocation. Using a combination of positional cloning and candidate gene approach, we cloned the breakpoint and were able to show that AF10 is fused to a novel gene that we named CALM (Clathrin Assembly Lymphoid Myeloid leukemia gene) located at 11q14. AF10, a putative transcription factor, had recently been cloned as one of the fusion partners of MLL. CALM has a very high homology in its N-terminal third to the murine ap-3 gene which is one of the clathrin assembly proteins. The N-terminal region of ap-3 has been shown to bind to clathrin and to have a high-affinity binding site for phosphoinositols. The identification of the CALM/AF10 fusion gene in the widely used U937 cell line will contribute to our understanding of the malignant phenotype of this line.

Links

PubMed PMC39360

Keywords

Adaptor Proteins, Vesicular Transport; Amino Acid Sequence; Animals; Base Sequence; Cell Line; Chromosomes, Human, Pair 10/genetics; Chromosomes, Human, Pair 11/genetics; Cloning, Molecular; DNA Primers/genetics; DNA, Complementary/genetics; Humans; In Situ Hybridization, Fluorescence; Leukemia, Myeloid, Acute/genetics; Mice; Molecular Sequence Data; Monocytes/ultrastructure; Monomeric Clathrin Assembly Proteins; Nerve Tissue Proteins/genetics; Phosphoproteins/genetics; Sequence Homology, Amino Acid; Transcription Factors/genetics; Translocation, Genetic; Tumor Cells, Cultured

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:PICAL

involved_in

GO:0016192: vesicle-mediated transport

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PICAL

involved_in

GO:0065003: protein-containing complex assembly

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete


See also

References

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