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PMID:8636207

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Citation

Lupashin, VV, Hamamoto, S and Schekman, RW (1996) Biochemical requirements for the targeting and fusion of ER-derived transport vesicles with purified yeast Golgi membranes. J. Cell Biol. 132:277-89

Abstract

In order for secretion to progress, ER-derived transport vesicles must target to, and fuse with the cis-Golgi compartment. These processes have been reconstituted using highly enriched membrane fractions and partially purified soluble components. The functionally active yeast Golgi membranes that have been purified are highly enriched in the cis-Golgi marker enzymes alpha 1,6 mannosyltransferase and GDPase. Fusion of transport vesicles with these membranes requires both GTP and ATP hydrolysis, and depends on cytosolic and peripheral membrane proteins. At least two protein fractions from yeast cytosol are required for the reconstitution of ER-derived vesicle fusion. Soluble fractions prepared from temperature-sensitive mutants revealed requirements for the Ypt1p, Sec19p, Sly1p, Sec7p, and Uso1 proteins. A model for the sequential involvement of these components in the targeting and fusion reaction is proposed.

Links

PubMed PMC2120720

Keywords

Biological Markers; Biological Transport; Cell Fractionation/methods; Endoplasmic Reticulum/physiology; Endoplasmic Reticulum/ultrastructure; Fungal Proteins/analysis; Golgi Apparatus/physiology; Golgi Apparatus/ultrastructure; Intracellular Membranes/physiology; Intracellular Membranes/ultrastructure; Mannosyltransferases/analysis; Membrane Fusion; Microscopy, Electron; Organelles/physiology; Organelles/ultrastructure; Pyrophosphatases/analysis; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:SLY1

involved_in

GO:0048280: vesicle fusion with Golgi apparatus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SEC17

located_in

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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