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PMID:8612273

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Citation

Lewis, MJ and Pelham, HR (1996) SNARE-mediated retrograde traffic from the Golgi complex to the endoplasmic reticulum. Cell 85:205-15

Abstract

Operation of the secretory pathway in eukaryotic cells requires the selective docking and fusion of transport vesicles with the appropriate target organelle. This is mediated in part by integral membrane proteins termed v-SNAREs (on vesicles) and t-SNAREs (on the target membranes). We describe a novel yeast t-SNARE that resides on the endoplasmic reticulum and mediates retrograde traffic from the Golgi complex. Mutation of this protein prevents both the HDEL receptor and a membrane protein bearing a dibasic retrieval signal from recycling to the endoplasmic reticulum. Forward traffic is also blocked, but only indirectly. Comparison with other yeast mutants indicates that Sec21p (gamma-COP) and Sec20p (an endoplasmic reticulum membrane protein) are also involved primarily, if not exclusively, in retrograde transport.

Links

PubMed

Keywords

Biological Transport/physiology; Endoplasmic Reticulum/chemistry; Endoplasmic Reticulum/metabolism; Fungal Proteins/metabolism; Golgi Apparatus/chemistry; Golgi Apparatus/metabolism; Intracellular Membranes/chemistry; Intracellular Membranes/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Membrane Proteins/pharmacology; Microscopy, Electron; Molecular Sequence Data; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Qa-SNARE Proteins; Receptors, Peptide/metabolism; SNARE Proteins; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Vesicular Transport Proteins; Yeasts/cytology; Yeasts/metabolism; Yeasts/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:SEC20

involved_in

GO:0006890: retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SEC20

involved_in

GO:0048279: vesicle fusion with endoplasmic reticulum

ECO:0000305: curator inference used in manual assertion

GO:0005484
GO:0006890

P

Seeded From UniProt

complete

YEAST:UFE1

enables

GO:0005484: SNAP receptor activity

ECO:0000247: sequence alignment evidence used in manual assertion

UniProtKB:Q01590

F

Seeded From UniProt

complete

YEAST:UFE1

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:UFE1

located_in

GO:0016021: integral component of membrane

ECO:0000255: match to sequence model evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:UFE1

involved_in

GO:0006890: retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:UFE1

involved_in

GO:0048279: vesicle fusion with endoplasmic reticulum

ECO:0000305: curator inference used in manual assertion

GO:0005484
GO:0006890

P

Seeded From UniProt

complete


See also

References

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