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PMID:8557736

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Citation

Aitchison, JD, Blobel, G and Rout, MP (1995) Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport. J. Cell Biol. 131:1659-75

Abstract

To extend our understanding of the mechanism by which the nuclear pore complex (NPC) mediates macromolecular transport across the nuclear envelope we have focused on defining the composition and molecular organization of the yeast NPC. Peptide sequence analysis of a polypeptide with a M(r) of approximately 100,000 present in a highly enriched yeast NPC fraction identified a novel yeast nucleoporin we term Nup120p. Nup120p corresponds to the open reading frame (ORF) YKL057c identified by the yeast genome sequencing project. The ORF predicts a protein with a calculated molecular mass of 120.5 kD containing two leucine zipper motifs, a short coiled-coil region and limited primary sequence similarity to Nup133p. Nup120p was localized to the NPC using a protein A-tagged chimera in situ by indirect immunofluorescence microscopy. Deletion of the NUP120 gene caused clustering of NPCs at one side of the nuclear envelope, moderate nucleolar fragmentation and slower cell growth. Transfer of nup120 delta cells to 37 degrees C resulted in the nuclear accumulation of poly(A)+ mRNA, extensive fragmentation of the nucleolus, spindle defects, and cell death.

Links

PubMed PMC2120676

Keywords

Amino Acid Sequence; Base Sequence; Biological Markers; Biological Transport/genetics; Fluorescent Antibody Technique; Gene Deletion; Genes, Fungal/physiology; Microscopy, Electron; Molecular Sequence Data; Nuclear Envelope/chemistry; Nuclear Envelope/metabolism; Nuclear Envelope/ultrastructure; Nuclear Pore Complex Proteins; Nuclear Proteins/analysis; Nuclear Proteins/genetics; Nuclear Proteins/isolation & purification; RNA, Messenger/metabolism; Saccharomyces cerevisiae Proteins; Sensitivity and Specificity; Temperature; Yeasts/cytology; Yeasts/genetics; Yeasts/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:NU120

involved_in

GO:0006611: protein export from nucleus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:NU120

involved_in

GO:0006406: mRNA export from nucleus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:NU120

involved_in

GO:0051664: nuclear pore localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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