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PMID:8552679

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Citation

Jones, PG, Mitta, M, Kim, Y, Jiang, W and Inouye, M (1996) Cold shock induces a major ribosomal-associated protein that unwinds double-stranded RNA in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 93:76-80

Abstract

A 70-kDa protein was specifically induced in Escherichia coli when the culture temperature was shifted from 37 to 15 degrees C. The protein was identified to be the product of the deaD gene (reassigned csdA) encoding a DEAD-box protein. Furthermore, after the shift from 37 to 15 degrees C, CsdA was exclusively localized in the ribosomal fraction and became a major ribosomal-associated protein in cells grown at 15 degrees C. The csdA deletion significantly impaired cell growth and the synthesis of a number of proteins, specifically the derepression of heat-shock proteins, at low temperature. Purified CsdA was found to unwind double-stranded RNA in the absence of ATP. Therefore, the requirement for CsdA in derepression of heat-shock protein synthesis is a cold shock-induced function possibly mediated by destabilization of secondary structures previously identified in the rpoH mRNA.

Links

PubMed PMC40181

Keywords

Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Chaperonin 60/genetics; Cold Temperature; DEAD-box RNA Helicases; Electrophoresis, Gel, Two-Dimensional; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; HSP70 Heat-Shock Proteins/genetics; Molecular Sequence Data; Mutagenesis, Insertional; Nucleic Acid Conformation; RNA Helicases; RNA, Bacterial/chemistry; RNA, Double-Stranded/chemistry; RNA-Binding Proteins/chemistry; RNA-Binding Proteins/metabolism; Ribosomes/chemistry

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:DEAD

acts_upstream_of_or_within

GO:0070417: cellular response to cold

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:DEAD

acts_upstream_of_or_within

GO:0070417: cellular response to cold

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:DEAD

enables

GO:0003724: RNA helicase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:DEAD

involved_in

GO:0070417: cellular response to cold

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:DEAD

involved_in

GO:0070417: cellular response to cold

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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