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PMID:8423783

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Citation

Sprenger, F, Trosclair, MM and Morrison, DK (1993) Biochemical analysis of torso and D-raf during Drosophila embryogenesis: implications for terminal signal transduction. Mol. Cell. Biol. 13:1163-72

Abstract

Determination of anterior and posterior terminal structures of Drosophila embryos requires activation of two genes encoding putative protein kinases, torso and D-raf. In this study, we demonstrate that Torso has intrinsic tyrosine kinase activity and show that it is transiently tyrosine phosphorylated (activated) at syncytial blastoderm stages. Torso proteins causing a gain-of-function phenotype are constitutively tyrosine phosphorylated, while Torso proteins causing a loss-of-function phenotype lack tyrosine kinase activity. The D-raf gene product, which is required for Torso function, is identified as a 90-kDa protein with intrinsic serine/threonine kinase activity. D-Raf is expressed throughout embryogenesis; however, the phosphorylation state of the protein changes during development. In wild-type embryos, D-Raf is hyperphosphorylated at 1 to 2 h after egg laying, and thereafter only the most highly phosphorylated form is detected. Embryos lacking Torso activity, however, show significant reductions in D-Raf protein expression rather than major alterations in the protein's phosphorylation state. This report provides the first biochemical analysis of the terminal signal transduction pathway in Drosophila embryos.

Links

PubMed PMC359001

Keywords

3T3 Cells; Amino Acid Sequence; Animals; Base Sequence; DNA; Drosophila/embryology; Drosophila/genetics; Electrophoresis, Polyacrylamide Gel; Ligands; Mice; Molecular Sequence Data; Phosphorylation; Protein-Serine-Threonine Kinases/genetics; Protein-Tyrosine Kinases/genetics; Protein-Tyrosine Kinases/metabolism; Signal Transduction/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:KRAF1

enables

GO:0004674: protein serine/threonine kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:KRAF1

involved_in

GO:0008293: torso signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0003733

P

Seeded From UniProt

complete

DROME:KRAF1

involved_in

GO:0046777: protein autophosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:TORSO

enables

GO:0004714: transmembrane receptor protein tyrosine kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:TORSO

involved_in

GO:0008293: torso signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:TORSO

involved_in

GO:0038083: peptidyl-tyrosine autophosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:TSL

involved_in

GO:0008293: torso signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:TRUNK

involved_in

GO:0008293: torso signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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