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PMID:8423783
| Citation |
Sprenger, F, Trosclair, MM and Morrison, DK (1993) Biochemical analysis of torso and D-raf during Drosophila embryogenesis: implications for terminal signal transduction. Mol. Cell. Biol. 13:1163-72 |
|---|---|
| Abstract |
Determination of anterior and posterior terminal structures of Drosophila embryos requires activation of two genes encoding putative protein kinases, torso and D-raf. In this study, we demonstrate that Torso has intrinsic tyrosine kinase activity and show that it is transiently tyrosine phosphorylated (activated) at syncytial blastoderm stages. Torso proteins causing a gain-of-function phenotype are constitutively tyrosine phosphorylated, while Torso proteins causing a loss-of-function phenotype lack tyrosine kinase activity. The D-raf gene product, which is required for Torso function, is identified as a 90-kDa protein with intrinsic serine/threonine kinase activity. D-Raf is expressed throughout embryogenesis; however, the phosphorylation state of the protein changes during development. In wild-type embryos, D-Raf is hyperphosphorylated at 1 to 2 h after egg laying, and thereafter only the most highly phosphorylated form is detected. Embryos lacking Torso activity, however, show significant reductions in D-Raf protein expression rather than major alterations in the protein's phosphorylation state. This report provides the first biochemical analysis of the terminal signal transduction pathway in Drosophila embryos. |
| Links | |
| Keywords |
3T3 Cells; Amino Acid Sequence; Animals; Base Sequence; DNA; Drosophila/embryology; Drosophila/genetics; Electrophoresis, Polyacrylamide Gel; Ligands; Mice; Molecular Sequence Data; Phosphorylation; Protein-Serine-Threonine Kinases/genetics; Protein-Tyrosine Kinases/genetics; Protein-Tyrosine Kinases/metabolism; Signal Transduction/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
DROME:KRAF1 |
enables |
GO:0004674: protein serine/threonine kinase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
DROME:KRAF1 |
involved_in |
GO:0008293: torso signaling pathway |
ECO:0000316: genetic interaction evidence used in manual assertion |
FB:FBgn0003733 |
P |
Seeded From UniProt |
complete | |
|
DROME:KRAF1 |
involved_in |
GO:0046777: protein autophosphorylation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DROME:TORSO |
enables |
GO:0004714: transmembrane receptor protein tyrosine kinase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
DROME:TORSO |
involved_in |
GO:0008293: torso signaling pathway |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DROME:TORSO |
involved_in |
GO:0038083: peptidyl-tyrosine autophosphorylation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DROME:TSL |
involved_in |
GO:0008293: torso signaling pathway |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DROME:TRUNK |
involved_in |
GO:0008293: torso signaling pathway |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
See also
References
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