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PMID:8407802

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Citation

Ma, D, Cook, DN, Alberti, M, Pon, NG, Nikaido, H and Hearst, JE (1993) Molecular cloning and characterization of acrA and acrE genes of Escherichia coli. J. Bacteriol. 175:6299-313

Abstract

The DNA fragment containing the acrA locus of the Escherichia coli chromosome has been cloned by using a complementation test. The nucleotide sequence indicates the presence of two open reading frames (ORFs). Sequence analysis suggests that the first ORF encodes a 397-residue lipoprotein with a 24-amino-acid signal peptide at its N terminus. One inactive allele of acrA from strain N43 was shown to contain an IS2 element inserted into this ORF. Therefore, this ORF was designated acrA. The second downstream ORF is predicted to encode a transmembrane protein of 1,049 amino acids and is named acrE. Genes acrA and acrE are probably located on the same operon, and both of their products are likely to affect drug susceptibilities observed in wild-type cells. The cellular localizations of these polypeptides have been analyzed by making acrA::TnphoA and acrE::TnphoA fusion proteins. Interestingly, AcrA and AcrE share 65 and 77% amino acid identity with two other E. coli polypeptides, EnvC and EnvD, respectively. Drug susceptibilities in one acrA mutant (N43) and one envCD mutant (PM61) have been determined and compared. Finally, the possible functions of these proteins are discussed.

Links

PubMed PMC206727

Keywords

Acriflavine/metabolism; Alkaline Phosphatase/genetics; Alkaline Phosphatase/metabolism; Alleles; Amino Acid Sequence; Anti-Bacterial Agents/toxicity; Bacterial Proteins/biosynthesis; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Base Sequence; Biological Transport; Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology; Carrier Proteins; Cloning, Molecular; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Genes, Bacterial; Genetic Complementation Test; Lipoproteins/biosynthesis; Lipoproteins/genetics; Membrane Proteins/biosynthesis; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Transport Proteins; Microbial Sensitivity Tests; Molecular Sequence Data; Multidrug Resistance-Associated Proteins; Open Reading Frames; Plasmids; Restriction Mapping; Sequence Homology, Amino Acid; Streptomycin/toxicity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:ACRA

located_in

GO:0030288: outer membrane-bounded periplasmic space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:ACRA

acts_upstream_of_or_within

GO:0042908: xenobiotic transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:ACRA

acts_upstream_of_or_within

GO:0046677: response to antibiotic

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:ACRE

located_in

GO:0031226: intrinsic component of plasma membrane

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:ACRE

acts_upstream_of_or_within

GO:0009410: response to xenobiotic stimulus

ECO:0000250: sequence similarity evidence used in manual assertion

EcoliWiki:AcrF

P

Seeded From UniProt

complete

ECOLI:ACRB

located_in

GO:0030288: outer membrane-bounded periplasmic space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:ACRB

located_in

GO:0005887: integral component of plasma membrane

ECO:0000245: automatically integrated combinatorial evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:ENVC

acts_upstream_of_or_within

GO:0009410: response to xenobiotic stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:ACRB

part_of

GO:0005887: integral component of plasma membrane

ECO:0000245: automatically integrated combinatorial evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:ACRB

part_of

GO:0005887: integral component of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:ACRB

enables

GO:0015238: drug transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:ACRB

involved_in

GO:0042493: response to drug

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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