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PMID:8392016

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Citation

Song, Q, Khanna, KK, Lu, H and Lavin, MF (1993) Cloning and characterization of a human protein phosphatase 1-encoding cDNA. Gene 129:291-5

Abstract

While sequence information is available for a number of eukaryotic protein phosphatase 1 (PP1)-encoding genes, the cloning and characterization of a complete human pp1 gene has not been reported. We have used two conserved regions within the pp1 family of genes to synthesize oligodeoxyribonucleotide primers for the amplification of a 438-bp sequence from human mRNA. This DNA fragment was sequenced to verify that it corresponded to a pp1 cDNA and it was used to screen a human cDNA library to isolate a full-length clone. The deduced amino acid (aa) sequence identified a protein of 330 aa in length. Comparison with the rabbit pp1 cDNA sequence showed some nucleotide differences, largely at the third position of the codon, with complete concordance at the aa level. Northern blot analysis revealed an mRNA of approximately 1.6 kb.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Cloning, Molecular; Conserved Sequence; Humans; Molecular Sequence Data; Oligonucleotide Probes; Phosphoprotein Phosphatases/chemistry; Phosphoprotein Phosphatases/genetics; Protein Phosphatase 1; RNA, Messenger/analysis; Rabbits; Sequence Alignment; Sequence Analysis, DNA

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:PP1A

enables

GO:0004722: protein serine/threonine phosphatase activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PP1A

involved_in

GO:0006470: protein dephosphorylation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete


See also

References

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