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PMID:8378310

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Citation

Sakurai, H, Hiraoka, Y and Fukasawa, T (1993) Yeast GAL11 protein is a distinctive type transcription factor that enhances basal transcription in vitro. Proc. Natl. Acad. Sci. U.S.A. 90:8382-6

Abstract

The yeast auxiliary transcription factor GAL11, a candidate for the coactivator, was partially purified from yeast cells, and its function was characterized in a cell-free transcription system. The partially purified GAL11 protein stimulated basal transcription from the CYC1 core promoter by a factor of 4-5 at the step of preinitiation complex formation. GAL11 protein also enhanced transcription activated by general regulatory factor 1, GAL4-AH, or GAL4-VP16 to the same extent as the basal transcription. Therefore, the apparent potentiation of the activators by GAL11 was attributable to the stimulation of basal transcription. The wild-type GAL11 protein (but not a mutant-type protein) produced in bacteria stimulated transcription as effectively as GAL11 from yeast. These results suggest that GAL11 functions as a positive cofactor of basal and activator-induced transcription in a cell-free transcription system.

Links

PubMed PMC47360

Keywords

Cell Nucleus/metabolism; DNA, Fungal/metabolism; Fungal Proteins/biosynthesis; Fungal Proteins/isolation & purification; Fungal Proteins/metabolism; Gene Expression Regulation, Fungal; Genes, Fungal; Immunoblotting; Kinetics; Mediator Complex; Mutagenesis; Plasmids; Promoter Regions, Genetic; Restriction Mapping; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; TATA Box; Trans-Activators; Transcription Factors/biosynthesis; Transcription Factors/isolation & purification; Transcription Factors/metabolism; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:MED15

enables

GO:0003713: transcription coactivator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:MED15

involved_in

GO:0051123: RNA polymerase II preinitiation complex assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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