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PMID:8376594
| Citation |
Donnenberg, MS, Tacket, CO, James, SP, Losonsky, G, Nataro, JP, Wasserman, SS, Kaper, JB and Levine, MM (1993) Role of the eaeA gene in experimental enteropathogenic Escherichia coli infection. J. Clin. Invest. 92:1412-7 |
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| Abstract |
Enteropathogenic Escherichia coli (EPEC) infections are a leading cause of infant diarrhea in developing countries. Recently eaeA, a gene necessary for the characteristic intimate attachment of EPEC to epithelial cells in tissue culture, was described. We conducted a randomized, double-blind study to determine the role of the eaeA gene in human EPEC infection. 11 adult volunteers ingested 2 x 10(10) colony-forming units of O127:H6 EPEC strain E2348/69, and an equal number received the same dose of an isogenic eaeA deletion mutant constructed from E2348/69. Volunteers were monitored for the development of diarrhea, fever, and systemic and gastrointestinal complaints. Diarrhea developed in all 11 volunteers who received E2348/69 and in 4 of 11 who received the mutant (P = 0.002). Fever was more common in recipients of the wild-type strain (P = 0.024). Stool volumes were lower in recipients of the mutant. All volunteers seroconverted to E2348/69 LPS, but the geometric mean peak titers of serum IgG and IgA in recipients of the mutant were lower than those of recipients of the wild-type strain. IgA against LPS was detected in the jejunal fluid of six of six recipients of E2348/69 and 5/6 recipients of the mutant. This study unambiguously assigns a role for eaeA as an EPEC virulence gene, but the residual diarrhea seen in recipients of the mutant indicates that other factors are involved. |
| Links |
PubMed PMC288285 Online version:10.1172/JCI116717 |
| Keywords |
Adhesins, Bacterial; Adult; Antibodies, Bacterial/biosynthesis; Bacterial Adhesion; Bacterial Outer Membrane Proteins; Carrier Proteins; Diarrhea/microbiology; Double-Blind Method; Escherichia coli/genetics; Escherichia coli/pathogenicity; Escherichia coli Infections/immunology; Escherichia coli Infections/microbiology; Escherichia coli Proteins; Genes, Bacterial; Humans; Sequence Deletion |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0009405: pathogenesis |
ECO:0000315: |
P |
Table 1. shows that lower frequency of developing diarrhea, vomiting, malaise, and anorexia for individuals who received eaeA deletion mutant in comparison to wild-type. |
complete | ||||
|
involved_in |
GO:0009405: pathogenesis |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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