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PMID:8300755

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Citation

Zhuang, YH, Landers, JP, Schuchard, MD, Syvälä, H, Gosse, B, Ruesink, T, Spelsberg, TC and Tuohimaa, P (1993) Immunohistochemical localization of the avian progesterone receptor and its candidate receptor binding factor (RBF-1). J. Cell. Biochem. 53:383-93

Abstract

An avian oviduct nuclear matrix protein in the 6-10 kDa size range has been implicated to function in the cell-free nuclear binding of the avian oviduct progesterone receptor (PR). This protein, termed the receptor binding factor-1 (RBF-1), has been purified and partially characterized [Schuchard et al.: Biochemistry 30:4535-4542, 1991]. This paper describes the immunohistochemical co-localization of the RBF-1 and PR in the avian oviduct cell nuclei and rat reproductive cell nuclei using antibodies directed specifically against the RBF-1 and activated PR. In the undifferentiated oviduct, the immunoreactivities for both PR and RBF-1 were co-localized in the nuclei of only epithelial cells, but not the stromal cells or smooth muscle cells. In the partially differentiated oviduct of estrogen treated chicks, the immunoreactivity co-localized in the nuclei of not only epithelial but also glandular and stromal cells. Staining for the PR, but not RBF-1, was detected in the smooth muscle cells. The intensity of the PR but not the RBF-1 staining was markedly down-regulated in these cells at 2 and 6 h after treatment of the animals with progesterone (P). However, the band patterns for RBF-1 in the Western blots did show qualitative changes which may reflect P-induced posttranslational modifications which alter the epitope on the RBF-1. Interestingly, immunohistochemical analysis of several reproductive tissues of the rat showed that certain cell types in the uterus, ovary, and prostate displayed strong positive nuclear staining for an RBF-1-like antigen(s).(ABSTRACT TRUNCATED AT 250 WORDS)

Links

PubMed Online version:10.1002/jcb.240530416

Keywords

Amino Acid Sequence; Animals; Antibodies, Monoclonal; Avian Proteins; Blotting, Western; Carrier Proteins/analysis; Cell Nucleus/chemistry; Chickens; Chromatin/chemistry; DNA-Binding Proteins/analysis; Epithelium/chemistry; Estradiol/pharmacology; Female; Immunohistochemistry; Intracellular Signaling Peptides and Proteins; Molecular Sequence Data; Oviducts/chemistry; Oviducts/ultrastructure; Rats; Receptors, Progesterone/analysis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:PRGR

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0004804)
  • part_of:(UBERON:0004245)
  • part_of:(CL:0002132)

Seeded From UniProt

complete

CHICK:Q7LZ14

involved_in

GO:0043627: response to estrogen

ECO:0000314: direct assay evidence used in manual assertion

P

  • part_of:(CL:0002132)

Seeded From UniProt

complete

CHICK:Q7LZ14

located_in

GO:0000785: chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0004804)
  • part_of:(CL:0002132)

Seeded From UniProt

complete

CHICK:Q7LZ14

involved_in

GO:0032570: response to progesterone

ECO:0000314: direct assay evidence used in manual assertion

P

  • part_of:(CL:0002132)

Seeded From UniProt

complete

CHICK:PRGR

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0004804)
  • part_of:(UBERON:0004245)
  • part_of:(CL:0002132)

Seeded From UniProt

complete

CHICK:PRGR

involved_in

GO:0032570: response to progesterone

ECO:0000314: direct assay evidence used in manual assertion

P

  • part_of:(CL:0002132)
  • part_of:(UBERON:0004245)

Seeded From UniProt

complete

CHICK:PRGR

involved_in

GO:0043627: response to estrogen

ECO:0000314: direct assay evidence used in manual assertion

P

  • part_of:(CL:0002132)
  • part_of:(UBERON:0004245)

Seeded From UniProt

complete

CHICK:Q7LZ14

involved_in

GO:0032570: response to progesterone

ECO:0000314: direct assay evidence used in manual assertion

P

part_of:(CL:0002132)

Seeded From UniProt

complete

CHICK:Q7LZ14

involved_in

GO:0043627: response to estrogen

ECO:0000314: direct assay evidence used in manual assertion

P

part_of:(CL:0002132)

Seeded From UniProt

complete

CHICK:Q7LZ14

part_of

GO:0000790: nuclear chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0004804)
  • part_of:(CL:0002132)

Seeded From UniProt

complete

Notes

See also

References

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