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PMID:8294495

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Citation

Crawford, AW, Pino, JD and Beckerle, MC (1994) Biochemical and molecular characterization of the chicken cysteine-rich protein, a developmentally regulated LIM-domain protein that is associated with the actin cytoskeleton. J. Cell Biol. 124:117-27

Abstract

LIM domains are present in a number of proteins including transcription factors, a proto-oncogene product, and the adhesion plaque protein zyxin. The LIM domain exhibits a characteristic arrangement of cysteine and histidine residues and represents a novel zinc binding sequence (Michelsen et al., 1993). Previously, we reported the identification of a 23-kD protein that interacts with zyxin in vitro (Sadler et al., 1992). In this report, we describe the purification and characterization of this 23-kD zyxin-binding protein from avian smooth muscle. Isolation of a cDNA encoding the 23-kD protein has revealed that it consists of 192 amino acids and exhibits two copies of the LIM motif. The 23-kD protein is 91% identical to the human cysteine-rich protein (hCRP); therefore we refer to it as the chicken cysteine-rich protein (cCRP). Examination of a number of chick embryonic tissues by Western immunoblot analysis reveals that cCRP exhibits tissue-specific expression. cCRP is most prominent in tissues that are enriched in smooth muscle cells, such as gizzard, stomach, and intestine. In primary cell cultures derived from embryonic gizzard, differentiated smooth muscle cells exhibit the most striking staining with anti-cCRP antibodies. We have performed quantitative Western immunoblot analysis of cCRP, zyxin, and alpha-actinin levels during embryogenesis. By this approach, we have demonstrated that the expression of cCRP is developmentally regulated.

Links

PubMed PMC2119904

Keywords

Amino Acid Sequence; Animals; Avian Proteins; Base Sequence; Cell Compartmentation; Chick Embryo; Chickens; Cloning, Molecular; Cytoskeletal Proteins/chemistry; Cytoskeletal Proteins/metabolism; DNA, Complementary; Fluorescent Antibody Technique; Gene Expression Regulation; Hot Temperature; Metalloproteins/genetics; Metalloproteins/isolation & purification; Microfilament Proteins/chemistry; Molecular Sequence Data; Molecular Weight; Muscle Proteins/chemistry; Muscle Proteins/genetics; Muscle Proteins/isolation & purification; Protein Structure, Tertiary; Proto-Oncogene Proteins c-myc/genetics; Proto-Oncogene Proteins c-myc/isolation & purification; RNA, Messenger/genetics; Sequence Alignment; Sequence Homology, Amino Acid; Tissue Distribution; Zyxin

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:ZYX

enables

GO:0051219: phosphoprotein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q04584

F

Seeded From UniProt

complete

CHICK:CSRP1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:CSRP1

located_in

GO:0005925: focal adhesion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:CSRP1

located_in

GO:0001725: stress fiber

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:ZYX

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P67966

F

Seeded From UniProt

complete

CHICK:ZYX

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:ZYX

located_in

GO:0005925: focal adhesion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:ZYX

located_in

GO:0001725: stress fiber

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:ACTN1

enables

GO:0051219: phosphoprotein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q04584

F

Seeded From UniProt

complete

CHICK:ACTN1

part_of

GO:0005623: cell

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:CSRP1

part_of

GO:0005925: focal adhesion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:CSRP1

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:CSRP1

part_of

GO:0001725: stress fiber

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:CSRP1

enables

GO:0051219: phosphoprotein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q04584

F

Seeded From UniProt

complete

CHICK:CSRP1

part_of

GO:0005623: cell

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0011903)
  • part_of:(UBERON:0004222)
  • part_of:(UBERON:0004221)

Seeded From UniProt

complete

CHICK:ZYX

enables

GO:0051393: alpha-actinin binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P05094

F

Seeded From UniProt

complete

CHICK:ZYX

part_of

GO:0001725: stress fiber

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:ZYX

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:ZYX

part_of

GO:0005925: focal adhesion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:ZYX

part_of

GO:0005623: cell

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0011903)

Seeded From UniProt

complete

CHICK:ZYX

enables

GO:0051393: alpha-actinin binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P05094-2

F

Seeded From UniProt

complete

Notes

See also

References

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