GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:8293872

From GONUTS
Jump to: navigation, search
Citation

Kispert, A and Herrmann, BG (1994) Immunohistochemical analysis of the Brachyury protein in wild-type and mutant mouse embryos. Dev. Biol. 161:179-93

Abstract

The murine Brachyury (T) gene is required in posterior mesoderm formation and axial development. Mutant embryos lacking T gene function are deficient in notochord differentiation and posterior mesoderm formation, but make anterior mesoderm. Posterior axial development requires increasing T activity along the rostrocaudal axis. The T gene is transiently transcribed in nascent and migrating mesoderm and continuously in the notochord. The maintenance of T expression in the notochord depends, directly or indirectly, on wild-type T activity. In Xenopus it has been shown that the onset of T expression occurs in response to mesoderm-inducing growth factors. The T protein is binding to DNA and is probably involved in the control of gene expression. Here we show that the T protein is located in the nucleus. We have analyzed the expression pattern of T protein in wild-type and mutant embryos from early primitive streak formation to the end of the tail bud stage. Throughout all stages of mesoderm formation T protein is transiently present in nascent and migrating mesoderm. In the notochord T protein persists to the end of the tail bud stage. It is also transiently detectable in the forming gut endoderm and in prospective neuroectoderm of later embryos. This shows that T expression is not strictly correlated with a commitment of cells to mesoderm. The analysis of the tail development of TWis/+ mutant embryos demonstrated that the formation of the neural tube, gut, and somites from the tail bud proceeds in the absence of a notochord. The maintenance and differentiation of these structures, however, seems to depend on signals from the notochord.

Links

PubMed Online version:10.1006/dbio.1994.1019

Keywords

Animals; Base Sequence; Cell Nucleus/chemistry; DNA-Binding Proteins/analysis; DNA-Binding Proteins/physiology; Embryo, Mammalian/chemistry; Embryonic and Fetal Development; Female; Fetal Proteins/analysis; Fetal Proteins/physiology; Immunohistochemistry; Mice; Molecular Sequence Data; Mutation; Pregnancy; T-Box Domain Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:TBXT

acts_upstream_of_or_within

GO:0001756: somitogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857760

P

Seeded From UniProt

complete

MOUSE:TBXT

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:TBXT

acts_upstream_of_or_within

GO:0007498: mesoderm development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857760

P

  • results_in_development_of:(EMAPA:16039)

Seeded From UniProt

complete

MOUSE:TBXT

acts_upstream_of_or_within

GO:0030903: notochord development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857760

P

  • results_in_development_of:(EMAPA:16039)

Seeded From UniProt

complete

MOUSE:TBXT

acts_upstream_of_or_within

GO:0048706: embryonic skeletal system development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857760

P

  • results_in_development_of:(EMAPA:16039)|results_in_development_of:(EMAPA:36588)|results_in_development_of:(EMAPA:19541)|results_in_development_of:(EMAPA:16039)|results_in_development_of:(EMAPA:36614)|results_in_development_of:(EMAPA:19591)

Seeded From UniProt

complete

MOUSE:TBXT

acts_upstream_of_or_within

GO:0036342: post-anal tail morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857760

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.