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PMID:8195289

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Citation

Dahl, SC, Geib, RW, Fox, MT, Edidin, M and Branton, D (1994) Rapid capping in alpha-spectrin-deficient MEL cells from mice afflicted with hereditary hemolytic anemia. J. Cell Biol. 125:1057-65

Abstract

A spectrin-based membrane skeleton is important for the stability and organization of the erythrocyte. To study the role of spectrin in cells that possess complex cytoskeletons, we have generated alpha-spectrin-deficient erythroleukemia cell lines from sph/sph mice. These cells contain beta-spectrin, but lack alpha-spectrin as determined by immunoblot and Northern blot analyses. The effects of alpha-spectrin deficiency are apparent in the cells' irregular shape and fragility in culture. Capping of membrane glycoproteins by fluorescent lectin or antibodies occurs more rapidly in sph/sph than in wild-type erythroleukemia cells, and the caps appear more concentrated. The data support the idea that spectrin plays an important role in organizing membrane structure and limiting the lateral mobility of integral membrane glycoproteins in cells other than mature erythrocytes.

Links

PubMed PMC2120060

Keywords

Animals; Cell Membrane/metabolism; Cell Membrane/ultrastructure; Friend murine leukemia virus; H-2 Antigens/metabolism; Immunologic Capping; Leukemia, Erythroblastic, Acute; Mice; Mice, Mutant Strains; Spectrin/deficiency; Spectrin/physiology; Time Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:SPTA1

acts_upstream_of_or_within

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1856377

P

Seeded From UniProt

complete

MOUSE:SPTA1

acts_upstream_of_or_within

GO:0008360: regulation of cell shape

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1856377

P

Seeded From UniProt

complete


See also

References

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