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PMID:8187880

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Citation

Loeber, G, Dworkin, MB, Infante, A and Ahorn, H (1994) Characterization of cytosolic malic enzyme in human tumor cells. FEBS Lett. 344:181-6

Abstract

Cytosolic NADP(+)-dependent malic enzyme (ME) from human tumor cells was characterized in detail and compared to ME from normal human tissues produced in recombinant E. coli. Kinetic properties, size as seen in SDS gels, and HPLC elution profiles of tryptic digests of human 'normal cell' ME and NADP(+)-ME from tumor cells were identical. Thus, NADP(+)-ME found in tumor cells does not constitute a tumor-specific isoform as suggested by other studies but is identical to the 'housekeeping protein' predominantly expressed in human liver and white adipose tissue.

Links

PubMed

Keywords

Adipose Tissue/enzymology; Amino Acid Sequence; Base Sequence; Chromatography, High Pressure Liquid; Colonic Neoplasms/enzymology; Cytosol/enzymology; Escherichia coli/genetics; Gene Expression; Gene Transfer Techniques; Humans; Isoenzymes/chemistry; Isoenzymes/metabolism; Kinetics; Liver/enzymology; Malate Dehydrogenase/chemistry; Malate Dehydrogenase/genetics; Malate Dehydrogenase/metabolism; Molecular Sequence Data; NADP/pharmacology; Neoplasms/enzymology; Peptide Fragments/chemistry; Peptide Fragments/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Trypsin/metabolism; Tumor Cells, Cultured

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:MAOX

enables

GO:0004470: malic enzyme activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P48163

F

Seeded From UniProt

complete

MOUSE:MAOX

enables

GO:0004473: malate dehydrogenase (decarboxylating) (NADP+) activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P48163

F

Seeded From UniProt

complete

MOUSE:MAOX

involved_in

GO:0006108: malate metabolic process

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P48163

P

Seeded From UniProt

complete

HUMAN:MAOX

involved_in

GO:0009165: nucleotide biosynthetic process

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

RAT:MAOX

enables

GO:0004470: malic enzyme activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P48163

F

Seeded From UniProt

complete

RAT:MAOX

enables

GO:0004473: malate dehydrogenase (decarboxylating) (NADP+) activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P48163

F

Seeded From UniProt

complete

RAT:MAOX

involved_in

GO:0006108: malate metabolic process

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P48163

P

Seeded From UniProt

complete

HUMAN:MAOX

involved_in

GO:0009743: response to carbohydrate

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MAOX

involved_in

GO:0006741: NADP biosynthetic process

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MAOX

enables

GO:0004470: malic enzyme activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MAOX

enables

GO:0004473: malate dehydrogenase (decarboxylating) (NADP+) activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MAOX

involved_in

GO:0006108: malate metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MAOX

involved_in

GO:0005975: carbohydrate metabolic process

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

See also

References

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