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PMID:8166682
| Citation |
Jehn, B, Chicaiza, G, Martin, F and Jaggi, R (1994) Isolation of three novel POU-domain containing cDNA clones from lactating mouse mammary gland. Biochem. Biophys. Res. Commun. 200:156-62 |
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| Abstract |
Using a PCR-based cloning strategy to identify putative members of the POU-family of transcription factors from genomic mouse DNA or from cDNA derived from lactating or involuting mouse mammary gland 56 POU-domain containing DNA fragments were isolated. Within these 56 clones three cDNA clones seem to be novel putative members of this transcription factor family, referred to as mPit-1R, mBrn-3R and MM-POU-III-A. Expression pattern studies were performed using a reverse transcriptase-mediated PCR approach. For all three different clones distinct developmental and tissue specific transcript levels were obtained, suggesting a tissue specific function of these newly isolated putative members of the POU-family of transcription factors. |
| Links |
PubMed Online version:10.1006/bbrc.1994.1428 |
| Keywords |
Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA Primers; DNA, Complementary/isolation & purification; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/genetics; Lactation/metabolism; Liver/metabolism; Mammary Glands, Animal/metabolism; Mice; Mice, Inbred Strains; Molecular Sequence Data; POU Domain Factors; Polymerase Chain Reaction; Sequence Homology, Amino Acid; Templates, Genetic; Transcription Factors/biosynthesis; Transcription Factors/genetics; Transcription, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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