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PMID:8163554

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Citation

Mulholland, J, Preuss, D, Moon, A, Wong, A, Drubin, D and Botstein, D (1994) Ultrastructure of the yeast actin cytoskeleton and its association with the plasma membrane. J. Cell Biol. 125:381-91

Abstract

We characterized the yeast actin cytoskeleton at the ultrastructural level using immunoelectron microscopy. Anti-actin antibodies primarily labeled dense, patchlike cortical structures and cytoplasmic cables. This localization recapitulates results obtained with immunofluorescence light microscopy, but at much higher resolution. Immuno-EM double-labeling experiments were conducted with antibodies to actin together with antibodies to the actin binding proteins Abp1p and cofilin. As expected from immunofluorescence experiments, Abp1p, cofilin, and actin colocalized in immuno-EM to the dense patchlike structures but not to the cables. In this way, we can unambiguously identify the patches as the cortical actin cytoskeleton. The cortical actin patches were observed to be associated with the cell surface via an invagination of plasma membrane. This novel cortical cytoskeleton-plasma membrane interface appears to consist of a fingerlike invagination of plasma membrane around which actin filaments and actin binding proteins are organized. We propose a possible role for this unique cortical structure in wall growth and osmotic regulation.

Links

PubMed PMC2120025

Keywords

Actins/metabolism; Actins/ultrastructure; Cell Cycle; Cell Membrane/metabolism; Cell Membrane/ultrastructure; Cytoskeleton/ultrastructure; Endocytosis; Microfilament Proteins/metabolism; Microscopy, Fluorescence; Microscopy, Immunoelectron; Osmosis; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:ABP1

located_in

GO:0030479: actin cortical patch

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:ACT

located_in

GO:0030479: actin cortical patch

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:ACT

located_in

GO:0000142: cellular bud neck contractile ring

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:ACT

located_in

GO:0032432: actin filament bundle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:COFI

located_in

GO:0030479: actin cortical patch

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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